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{
"caption": "The effects of gentamicin and Cd2+ on cell membrane integrity. Animals were treated with either Cd2+ (0.6 mg/kg, sc 5 days a week for 6 weeks) or gentamicin (100 mg/kg ip per day for 8 days) and the left kidneys were infused with ethidium homodimer. Cryosections of the kidneys were then fixed, permeabilized and labeled with DAPI as described in the Methods section. Panels A-D are phase contrast images corresponding to DAPI-labeled panels E-H and ethidium homodimer labeled nuclei in panels I-L. Gentamicin treatment resulted in increased ethidium homodimer labeling (J) in the renal cortex as compared to control (I). No differences in ethidium homodimer labeling were detected in 6-week Cd2+-treated images (L) compared to control (K). The scale bar in the top left image represents 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1810561-2-1472-6793-7-1-7.jpg"
} | 000800 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The effects of Hg2+ on the general morphology of the outer renal cortex. Rats were treated with Hg2+ (0.9% NaCl vehicle control, 0.875 and 3.5 mg/kg, i.p.). 24 h later, the kidneys were removed, and processed for H & E staining. Panels A-C are representative sections from the non-perfused right kidney and panels D-F are representative sections from the perfused left kidney under high-power (HP). (G-I) are representative images of the perfused left kidney under low-power (LP). Panels A, D, G are from vehicle control treated animals, panels B, E, H are from 0.875 mg/kg Hg2+-treated animals and panels C, F, I are from 3.5 mg/kg Hg2+-treated animals. A white arrow in panels H and I represent the boundary between the renal cortex and the outer medulla. The scale bar in the top left image represents 100 μm and the scale bar in the bottom left image represents 500 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1810561-3-1472-6793-7-1-3.jpg"
} | 000801 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Effects of Hg2+ on ethidium-labeling in the renal cortex. Animals were treated with Hg2+ (3.5 mg/kg, ip) for 24 h and the left kidneys were infused with ethidium homodimer. Cryosections of the kidneys were then fixed, permeabilized and labeled with DAPI as described in the Methods section. Panels A, D, G are high-powered (HP) images from control animals and panels B, E, H are from 3.5 mg/kg Hg2+-treated animals. Panels C, F, I represent low-powered (LP) images from 3.5 mg/kg Hg2+-treated animals. Panels A-C show phase-contrast images of the same fields in D-F and G-I, respectively. Panels D-F show total nuclei labeled in each field by DAPI, G-I show labeled nuclei by ethidium homodimer. The white arrow in panel I indicates the boundary between inner medulla and the renal cortex. The scale bar in the top left image represents 100 μm and the scale bar in the top right image represents 500 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1810561-4-1472-6793-7-1-4.jpg"
} | 000802 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Dose-response for Hg2+-induced cell death. Animals were treated with Hg2+ (0.875 and 1.75 mg/kg, ip) for 24 h and the left kidneys were infused with ethidium homodimer. Cryosections of the kidneys were then fixed, permeabilized and labeled with DAPI as described in the Methods section. Panels A, D, G are images from 0.9% NaCl vehicle control-treated animals. Panels B, E, H are from 0.875 mg/kg Hg2+-treated animals, and panels C, F, I are from 1.75 mg/kg Hg2+-treated animals. Panels A-C show phase-contrast images of the same fields in D-F and G-I, respectively. D-F show total nuclei labeled in each field by DAPI fluorescence, G-I show nuclei labeled by ethidium homodimer. The scale bar in the top left image represents 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1810561-5-1472-6793-7-1-5.jpg"
} | 000803 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Screening and electron microscopy of bacteriophage 0305φ8-36. Bacteriophage 0305φ8-36 was initially propagated and isolated [17] from soil frequented by cattle at the King Ranch (Kingsville, Texas). The host was a locally isolated Bacillus that was typed as B. thuringiensis by sequencing of the gene for 16s ribosomal DNA, as previously described [17]. During isolation, single-plaque cloning was performed [17] in gels of 0.40%, 0.20% and 0.15% agarose. The inocula for all three Petri plates were bacteriophages from a single plaque of the previous propagation, transferred by sterile needle and then non-uniformly spread [17]. The three Petri plates were at the same temperature (±0.2 C) during incubation. Photographic images are shown of Petri plates used for propagation in agarose gels of the following percentages: (a) 0.4, (b) 0.20, (c) 0.15. (d) In a more comprehensive experiment, plots of plaque diameter as a function of agarose gel percentage were made for bacteriophages G, T4 and 0305φ8-36 (0305φ8-36 is abbreviated by 36 in the figure). The molten agarose solution was the same among the different bacteriophages in (d). The host for bacteriophage G was Bacillus megaterium; the host for T4 was Escherichia coli BB/1. All Petri plates for (d) were in contact with the same surface and the temperature did not vary among them by more than 0.2°C. (e) Electron microscopy was performed of bacteriophage 0305φ8-36 negatively stained with sodium phosphotungstate after purification from a plate stock by use of a cesium chloride step gradient [17]. The length of the bar is 0.1 μm; magnification calibration was checked with a diffraction grating. The tails of all bacteriophage particles have partially contracted. By this criterion, 0305φ8-36 is a myovirus.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817643-0-1743-422X-4-21-1.jpg"
} | 000804 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Immunofluorescence analysis of S2/ZF-ZR and S2/JEF-JER cells with antibody 2F2. S2 cells were transfected with pAc/ZF-ZR (frame A) or pAc/JEF-JER (frame B). Transfected cells were harvested in PBS and plated onto microscopic slides. After air drying, the cells were fixed in 10% formalin with 1% Triton X-100 at room temperature for 30 min. The cells were incubated subsequently with JEV E specific monoclonal antibody 2F2 and a fluorescein-conjugated secondary antibody (DAKO) at room temperature for 1 hr. Each incubation was followed by two washes in PBS. Finally the slides were mounted with VECTASHIELD Mounting Medium containing propidium iodide (Vector Laboratories) and viewed under an Olympus fluorescence microscope. The green fluorescence indicates the detection of JEV E proteins.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817644-4-1743-422X-4-17-1.jpg"
} | 000805 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Decrease in DC-LAMP+ dendritic cells in eruptive papules compared to psoriasis. (A) CD3+ and (B) DC-LAMP+ cells in psoriasis, showing abundant CD3+ cells in the epidermis and dermis, and clusters of DC-LAMP+ cells in the reticular dermis in lesional skin. (C) There is weak expression of DC-LAMP on only a few cells in a papular lesion.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817648-2-1471-5945-7-2-5.jpg"
} | 000806 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Immunohistochemistry of papular lesions from three representative patients. (A) Patient 5, (B) Patient 8, (C) Patient 10. Staining with H&E, keratin 16 (K16), CD3+ T cells, α chain of β2 integrins CD11a (blocked by efalizumab), CD11b, CD11c, iNOS, and neutrophil elastase. There are abundant CD11b, CD11c+, and iNOS+ cells in the lesions, with relatively less CD3+ lymphocytes.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817648-3-1471-5945-7-2-2.jpg"
} | 000807 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "OspB Complementation Restores B. burgdorferi Ability to Survive and Colonize TicksResults from the experiments with the ticks inoculated by microinjection via rectal aperture are shown. Approximately 103 spirochetes were microinjected into each tick.(A) Shown are representative confocal microscopic images of the blood meal and gut samples of microinjected nymphs at 48 h during feeding. Samples were stained with FITC-labeled anti-Borrelia antibody (green), and tick cell nucleic acid was stained with propidium iodide (red). Scale 20 μM.(B) Average value of number of spirochetes from 15 random microscopic field observations is shown.(C) Q-RT-PCR for the total RNA extracted from microinjected nymphs collected at 48 h during feeding is shown. Values on Y-axis represent pg flaB/μg tick β-actin cDNA.(D) Shown are the readings from an in vitro binding assay of the wild-type, the ospB mutant, and the OspB complemented mutant B. burgdorferi to TGE- and FBS-coated wells (see Materials and Methods for details). Values on Y-axis are sample absorbance measured at 450 nm of wavelength. The values shown are the averages of four independent experiments. Black, open, and gray bars in (B, C, and D) indicate values for the wild-type, the ospB mutant, and the OspB complemented mutant spirochetes, respectively. Error bars define standard deviation (+) from the average value (mean). * indicates values that are statistically significant (p < 0.05, Student t test) in comparison to the ospB mutant.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817655-4-ppatp0030033pg004.jpg"
} | 000808 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical Detection of PrP in Brains of C57Bl/6 Mice Following Second-Passage Transmission of BSE and BASE(A–E) Mice infected with mouse-derived BSE or (F–J) mouse-derived BASE show the same brain regional distribution and patterns of deposition of PrPSc. Micrographs were obtained from anterior-to-posterior coronal sections of the brain (A–C and F–H) and from corresponding areas of cerebral cortex (D and I) and cerebellum (E and J) probed with the anti-PrP antibody 6H4. Scale bar: 1 mm (A–C and F–H); 200 μm (D, E, I, and J).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817656-0-ppatp0030031pg006.jpg"
} | 000809 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Brain MRI of Tgbov XV Mice Infected with BSE and BASET2-weighted images of anterior-to-posterior coronal planes of (A–C) BASE-infected mouse; (D–F) BSE-infected mouse; (G–I) uninfected Tgbov XV mouse. Both BSE- and BASE-infected mice show high signal intensity in the septal region: arrowheads in (A) and (D); and cerebellum: arrowheads in (C) and (F) compared to control (G) and (I). In addition, mice challenged with BASE exhibit scattered hyperintense areas in frontal regions: arrows in (A); and midbrain: arrows in (B) that are absent in BSE-infected and uninfected mice.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817656-4-ppatp0030031pg002.jpg"
} | 000810 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Analysis of pNiiA-CPR Associated Morphological Terminal PhenotypesTerminal growth phenotypes of pNiiA-CPR mutants were observed under a microscope (×160) with conidia grown for 36 to 40 h at 30 °C under standard repressing conditions. A continuum of conidia germination phenotypes of high penetrance was observed; ranging from those completely failing to undergo polarized growth (SEC31, SLY1) or swollen and highly disorganized condidia (GFA1), to those displaying stunted (TUB1, ERG10) or nonbranching germlings with swollen conidia (HEM15) with only rudimentary polarized growth. Micromycelial colonies were observed for a pNiiA-FKS1 mutant and resembling the morphology of wild-type A. fumigatus when grown in the presence of minimum effective concentration (MEC) of the FKS1p inhibitor, caspofungin [21]. Growth phenotypes under inducing conditions are shown in Figure S3.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817658-4-ppatp0030024pg004.jpg"
} | 000811 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Photographs of (A) sunset on Lake Victoria (Kenya) showing a huge swarm of nonbiting midges moving over Rusinga Island toward the northern shore of the lake (photographed by H. Nadel), and (B) adult chironomids adherred to the engine cover of a car passing beside a sewage pond before sunrise (photographed by M. Broza).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1817714-10-ehp0115-000195f5.jpg"
} | 000812 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Immunohistochemical detection (on frozen tissue sections) of CD40L+ cells within UNPC microenvironment. CD40L positivity is manifested as dot-like staining on isolated small lymphocytes. A. The figure shows a high content of CD40L+ lymphocytes, either intermingled with tumor cells or located around UNPC tumor nests, in a case (female, 37 years old) with high serum levels of sCD40L. B. The figure shows few CD40L+ lymphocytes in a case (male, 34 years old) with a low content of sCD40L. Original magnification ×250.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819365-0-1750-9378-2-5-4.jpg"
} | 000813 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Detection of LMP-1 expression by immunohistochemistry (red) in UNPC tumor cells. Alkaline phosphatase anti-alkaline phosphatase, original magnification ×250.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819365-2-1750-9378-2-5-2.jpg"
} | 000814 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Microendoscopic assessment of the same area in \"Figure 6\" showing the surface view of a taste bud, with the nuclei and cell borders outlined by methylene blue dye. The mucosa is normal in appearance.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-0-1746-160X-3-13-7.jpg"
} | 000815 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Microendoscopic image (x150) of the surface of the white patch in \"Figure 8\" showing the typical background appearance of keratosis with deeper nuclei visible as streaks and Civatte bodies (indicative of Lichen Planus).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-1-1746-160X-3-13-9.jpg"
} | 000816 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Histopathological image of a biopsy guided by the assessment in \"Figure 9\" showing keratosis with scattered nuclei (20–40 cell layers deep) and Civatte bodies at the basement membrane conducive with a diagnosis of Lichen Planus. This is at right angles (i.e. transverse section) to the plane of assessment in \"Figure 9\".",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-10-1746-160X-3-13-10.jpg"
} | 000817 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Clinical photograph of an edentulous patient presenting with a sore white patch in the floor of the mouth. This is prior to methylene blue staining.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-2-1746-160X-3-13-8.jpg"
} | 000818 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Methylene blue staining of the dorsum of the tongue prior to mucosal mircoendoscopic assessment.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-4-1746-160X-3-13-6.jpg"
} | 000819 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Post sweep microendoscopic examination (x60) of buccal mucosa showing mucosal unruffling and the even spread of methylene blue stained nuclei which gives the tissue a normal appearance.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-5-1746-160X-3-13-5.jpg"
} | 000820 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Initial microendoscopic examination (x60) of buccal mucosa stained with methylene blue showing an area of high nuclear density visible as a streak. This is because at the microscopic level the epithelial surfaces are ruffled or concertinaed which distorts the image viewed and causes the impression of a high nuclear density per image field.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-8-1746-160X-3-13-3.jpg"
} | 000821 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Mid sweep microendoscopic examination (x60) of buccal mucosa showing mucosal unruffling and spread of methylene blue stained nuclei.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819375-9-1746-160X-3-13-4.jpg"
} | 000822 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Effect of FBXW7 overexpression in glioma cells. Immunodetection of FLAG-Fbxw7 (green) in U87, 48 hours after transfection with plasmids encoding each of the three isoforms α (A), β (B), γ (C). Nuclei are labeled with DAPI (blue) and actin cytoskeleton with rhodamin-coupled phalloidin (red), bar = 50 μm. α-Fbxw7 localizes to the nucleus, β-Fbxw7 is cytoplasmic, γ-Fbxw7 localization varies. It is restricted to the nucleolus at low levels (insert) and leaks in the nucleus and in the cytoplasm at higher doses. Example of PCNA detection (red) in U87 cells overexpressing FLAG-Fbxw7 (green) α (D), β (E), γ (F). In lower panels (G-I), only the red signal in the same fields is shown. An example of α-Fbxw7-positive PCNA-negative cell is shown with arrowhead. bar = 10 μm. Quantification analysis of PCNA expression 48 h after transfection (J). Scoring was established on >150 cells per assay. The results (mean of two independent experiments, ± SEM) are expressed as the ratio of PCNA+ cells in FLAG+ versus FLAG- cells from the same transfection well. Quantification analysis of Ki-67 expressing cells in FLAG- and FLAG+ cells 48 h after transfection (K). Statistical analysis was performed using the Fisher's exact test (n > 150 cells for each group). The experiment was repeated twice with similar results. Cell growth after transfection with each isoform expression plasmid (L). For each assay, cells were stained with DAPI and anti-FLAG antibody 24 h and 72 h after transfection. FLAG positive and negative cells were scored from 20 independent 40×-magnified fields and their 72 H/24 h ratio compared. Cells overexpressing nuclear isoforms α and γ are significantly counterselected. Statistical analysis was performed using the Fisher's exact test (n > 1000 cells for each assay). The experiment was repeated twice and analyzed once after 48 h with similar results.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819378-2-1747-1028-2-9-4.jpg"
} | 000823 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "preoperative aortography.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819379-5-1749-8090-2-15-1.jpg"
} | 000824 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Trans-thoracic ECO 18 months later. Wrapping does not tighten the aortic root which does not reach the diameter of 50 mm of the custom-made prosthesis.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819379-6-1749-8090-2-15-6.jpg"
} | 000825 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Influence of diet on vascularity in the CT-2A (A and B) and the U87 (C) brain tumors grown orthotopically in B6 or SCID mice, respectively. Vessels were stained with the Factor VIII antibody and each stained section was representative of the entire tumor. For quantitative analysis of vessels in the CT-2A tumor, microvessel density was expressed as the number of vessels/200 × high power field (hpf). The values are expressed as the mean of three independent samples with error bars representing SEM. The asterisks indicate that the number of vessels/hpf was significantly less in the KC-R group than in the SD-UR group at P < 0.05. The scale bar represents 250 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819381-4-1743-7075-4-5-5.jpg"
} | 000826 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Electron microscopy analysis of structure changes in E. coli challenged baboon lung. a: normal architecture of the alveolar septae in healthy baboons; b. accumulation of neutrophils (PMN) and the presence of intra-alveolar bleeding erythrocytes (arrow) can be observed at 2 hrs; c: the increased accumulation of macrophages, fibroblasts and collagen deposition at 24 hrs. av, alveola; coll, collagen; Fb, fibroblasts; Mac, macrophages; RBC, red blood cells. Magnification: ×7000.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_105-PMC1819384-3-1471-2164-8-58-5.jpg"
} | 000827 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Representative histology of May-Grunwald stained sections (×10) of the distal colon from healthy control mice (A), mice with acute TNBS-induced colitis that have been or not (B) administered with non-treated DC (C) and Lr32-treated DC (D) and corresponding Ameho scores (E), mean±SEM (number of mice n = 10).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819555-3-ponep0000313pg005.jpg"
} | 000828 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Internalization of L. rhamnosus by BMDCs. BMDCs were incubated for 18 h with Lr32 strain at a bacteria-to-DC ratio of 10 and then processed for transmission electron microscopy. Bacteria are visible within membrane-bound phagosomes at various stages of degradation (arrows). Bar, 2 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819555-6-ponep0000313pg001.jpg"
} | 000829 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A) Western blot confirmation of XIAP over-expression in injected eyes in P23H animals in comparison to the contralateral control uninjected eye in the same animal. Blot was probed with an anti-HA antibody. Given that the protein extracts were made from the whole retina, and only a fraction of the retina was covered by the subretinal injection, XIAP protein levels seen on the Western blot are an under-estimation of the level of over-expression at the site of injection. Some variability in XIAP expression is present between animals. Ponceau red staining of membrane confirms equal loading. (B) Anti-HA immunofluorescence (green) confirms XIAP over-expression at site of neuroprotection in a P23H animal. (C) Contralateral untreated control has no fluorescence and shows diminished ONL thickness. (D) GFP-injected retina of a S334ter animal is shown at boundary between photoreceptors covered by the virus and photoreceptors outside the range of the virus. ONL thickness is similar on either side of the virus boundary. Sections are counterstained with DAPI (nuclear stain).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819556-2-ponep0000314pg003.jpg"
} | 000830 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Structural protection of photoreceptors. A) Haematoxylin and Eosin staining of retinal sections at 28 weeks after AAV subretinal injection. XIAP-treated retinas have increased layers of photoreceptor nuclei in comparison to contralateral untreated controls as well as age-matched GFP-treated controls. B) For the LE-P23H animals, XIAP-treatment preserves the ONL in comparison to both the untreated control eyes (p<0.0001) and GFP-treated eyes (p<0.0001) (XIAP N = 16, GFP N = 4). For LE-S334ter, XIAP-treated eyes are similarly significantly better than untreated controls (p<0.001) and GFP-treated eyes (p<0.005) (XIAP N = 15, GFP N = 8).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819556-3-ponep0000314pg002.jpg"
} | 000831 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "EPI data quality.A representative example of raw EPI (echoplanar imaging) data normalized to MNI space is shown in a coronar (MNI y = −7 mm) and axial (MNI z = −20 mm) section. The axial section corresponds approx. to the sections shown in [51]. The example represents the average across all EPI volumes acquired during the experimental session of one subject. The outline of the amygdala is shown (red line), enclosing the area with at least 50% probability of belonging to the amygdala (according to the probabilistic maps from [26]). The extent of the laterobasal (LB) group of the amygdala (LB, > = 80% probability) is shown in blue, the superficial group (SF) in green, and the centromedial group (CM) in magenta. In addition, the outline of the segmentation mask enclosing the area with sufficient signal for application of point spread function (PSF) based EPI distortion correction (see reference [39] for further details) is shown in yellow. In all subjects, the whole analyzed extent of the amygdala was within the segmentation mask and therefore distortion correction was possible in this region. Good EPI signal quality in the amygdala region was achieved in all subjects investigated.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819558-0-ponep0000307pg001.jpg"
} | 000832 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Characterization of ceruloplasmin (Cp) expression following an acute innate immune challenge. (A) Hybridization signals of Cp riboprobe on brain sections of saline-, or LPS-, or LPS/RU486-treated mice, sacrificed 12 h after the intrastriatal injection. (B) Semi-quantitative analysis of Cp mRNA levels (O.D.) depicted in “A”. (C) Results of quantitative reverse-transcriptase PCR (qPCR). (D) RMA expression levels of four different probe sets to detect Cp transcript selected according to a significant LPS effect. (E) Representative microphotographs of labeling experiments combining immunohistochemistry with in situ hybridization. Silver grain signals (Cp mRNA) overlap IBA1-, GFAP- and CD31-positive cells. (F) Confocal microscopy results showing co-localization of CP protein with CD31-, IBA1- and GFAP-positive cells, but not with MAC-2-positive cells, in brain sections from animals sacrificed 24 h after LPS challenge. Dark arrowheads: co-localization of silver grains with immunolabeling brown staining. Empty white arrows: co-localization determined by confocal laser scanning. Full white arrows and white arrowheads were used to identify cell types that failed to show convincing co-localization. One-way ANOVA followed by a Tukey'S HSD multiple comparison test: significantly different (** p<0.01, *** p<0.005) from the saline-injected group; significantly different (# p<0.05, ### p<0.005) from the LPS-treated group. Abbreviations: bv, blood vessel; m, meningis.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819560-1-ponep0000310pg005.jpg"
} | 000833 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Modulation of cerebro-vascular response by ceruloplasmin during early phases of pneumococcal infection. (A) Survival curves of Cp+/+, +/- and -/- following inoculation with S. Penumoniae (2 week follow up). (B) Ceruloplasmin protein immunoreactivity at the vascular level in the hindbrain of a Cp+/+ infected mouse; Cp-/- mice was used as control for the primary antibody. (C) Dark-field photomicrographs showing representative hybridization signals of Nfkbia or Selp riboprobes on hindbrain sections of Cp+/+ or -/- mice killed at the early phase of infection (36–48 h post-infection). (D) High magnification photomicrographs depicting the expression pattern of Selp mRNA associated with large blood vessels and not in cells lining the meninges or the ventricle. (E) Semi-quantitative analysis of Nfkbia and Selp mRNA levels O.D. Student's t-test was used to compare Cp+/+ and Cp-/- means (see text for details); * p<0.05. Abbreviations: bv, blood vessel; Cb, cerebellum; IC, inferior colliculus; m, meninges.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819560-4-ponep0000310pg007.jpg"
} | 000834 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Ceruloplasmin regulates brain iron levels during inflammation and confers neuroprotection. (A) Cp+/+, +/- or -/- mice were sacrificed 2 days after LPS infusion and brain sections stained for Iron III (Perls' staining). (B) High magnification of iron labeling (representative brain sections from Cp-/-mice sacrificed 2 or 5 days after LPS infusion). (C) Surface analysis to determine extent of iron deposition. (D) Fluoro-Jade B (FJB) staining was performed in brain sections from Cp+/+, +/- and -/- infused with LPS or saline. Volumetric analysis was performed by stereological procedure. (E) Representative dark-field picture of adjacent brain sections hybridized with Tlr2 cRNA probe in LPS treated mice 2 days after infusion. (F) Semi-quantitative analysis of Tlr2 mRNA hybridization signal. (G) Representative sections hybridized with Plp cRNA riboprobe to detect regions lacking signals (demyelinated area). These panels show two different coronal levels (cannulae track level and a section distal to the site of injection). (H) Surface analysis of demyelinated area. (I) and (J) are representative microphotographs of Sudam Black B (SBB) stained sections from Cp+/+ and -/- mice sacrificed 2 days after LPS infusion, as indicated. Statistical analysis was performed via a two or three-way ANOVA followed by a Bonferroni's multiple comparison test when applicable. “a”, significant genotype effect (p<0.05), significant time effect (p<0.01), significant treatment effect (p<0.001), Cp-/-significantly different from Cp+/+ (p<0.01) and Cp+/- (p<0.05). “b”, significant genotype effect (p<0.01), Cp-/- statistically different from Cp+/+ (p<0.005). “c”, significant interaction between treatment and time factors (p<0.01), LPS treatment 2 days statistically different from LPS 5 days (p<0.001). “d”, significant interaction between treatment and genotype effect (p<0.05), significant time effect (p<0.001), in the LPS treated group Cp-/- was statistically different from Cp+/+ († p<0.005) and from Cp+/- (‡ p<0.01). Abbreviations: cc, corpus callosum; CPu, caudate putamen; ct, cannulae track; Cx, cortex; LV, lateral ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1819560-5-ponep0000310pg006.jpg"
} | 000835 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A silicone breast implant in this patient caused marked limitation of echocardiographic acoustic window and image acquisition in the parasternal long axis view obscuring left ventricular cavity and septum.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820591-0-1476-7120-5-9-1.jpg"
} | 000836 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The arrow shows a large shadow and a bright ring obscuring the subcostal acoustic window.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820591-1-1476-7120-5-9-4.jpg"
} | 000837 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Similar to figure 2, a large shadow is seen across the right and left ventricle secondary to the silicone breast implant in this patient limiting echocardiographic window in the 4 chamber view.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820591-2-1476-7120-5-9-3.jpg"
} | 000838 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A large shadow is seen across the right and left ventricle caused by the silicone breast implant that limited the echocardiographic window in the 4 chamber view.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820591-3-1476-7120-5-9-2.jpg"
} | 000839 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "In order to improve the acoustic window, the parasternal long axis view was modified by the echocardiographer causing tilting of the image.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820591-4-1476-7120-5-9-5.jpg"
} | 000840 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Computed tomography showing well-defined, round mass in the right breast (arrow).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820595-0-1477-7819-5-25-2.jpg"
} | 000841 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Photomicrograph showing islands of tumour cells with clear cytoplasm, lying in fibrovascular stroma (Hematoxylin & Eosin, 10×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820595-1-1477-7819-5-25-4.jpg"
} | 000842 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Computed tomography showing tumour mass in the renal bed (arrow points to surgical clips).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820595-2-1477-7819-5-25-3.jpg"
} | 000843 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Differential extraction of CLIC1 from Panc1 Cells with digitonin and Triton X-100. Panc1 cells grown on glass coverslips were extracted with 0.004% (w/v) digitonin (A) or 0.5% (v/v) Triton X-100 (B) prior to fixation and staining for cytokeratin (green) and CLIC1 (red). The scale bar in each panel represents 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-1-1471-2121-8-8-7.jpg"
} | 000844 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Effect of digitonin extraction on distribution of CLIC1 in Panc1 cells. Panc1 cells were fixed with PLP without (A, C) or with (B, D) prior extraction with digitonin. Cells were then stained with AP1089 (A, B) or with both AP1089 and 9F5 (C, D) and imaged using confocal microscopy. In A and B, images are shown from the very base of the cell (left panel) plus images at focal planes 2 (center) and 4 (right) μm higher. Collection of images in B required higher sensitivity than A. Parallel cultures stained with control antisera and imaged under identical conditions were blank (not shown). In panels C and D, cells were double stained with AP1089 with Alexafluor565-conjugated goat anti-rabbit IgG (red, left panel) and 9F5 with Alexafluor488-conjugated goat anti-mouse IgG (green, center panel) without (C) or following (D) digitonin extraction. A merged image for each pair is shown on the right. Scale bar in A and B represent 25 μm, scale bars in C and D represent 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-10-1471-2121-8-8-4.jpg"
} | 000845 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Staining of mouse frozen tissue sections for CLIC1. Images are arranged as in figure 2. A, B. Pancreas with 10× objective. Bar = 100 μm. C. Pancreatic duct from B with 40× objective. Bar = 20 μm. D, E. Lung with 4× objective. Bar = 200 μm. F. Airway epithelium as in E with 60× objective. Bar = 20 μm. G, H. Head of epididymis with 10× objective. Bar = 100 μm. I. Epithelial layer from head of epididymis as in H with 60× objective. Bar = 20 μm. J, K. Tail of epididymis with 10× objective. Bar = 100 μm. L. Tail of epididymis as in K with 40× objective. Bar = 20 μm. M, N. Skeletal muscle under 20× objective. Bar = 50 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-11-1471-2121-8-8-3.jpg"
} | 000846 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Colocalization of CLIC1 with megalin and NaPi-II in MPTC cells. MPTC cells were grown on collagen-coated filters, fixed, permeabilized, and stained for CLIC1 with the 9F5 monoclonal antibody (green, center column) and two markers of the apical endocytic/recycling compartment (red, left column). The right column in each set is a merged image generated from the red and green channels. A. Two separate fields from near the apical pole of the cells stained for megalin (red) and CLIC1 (green). B. Two separate field from near the apical pole of the cells stained for NaPi-II (red) and CLIC1 (green). The scale bars represent 10 microns in each set of images.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-12-1471-2121-8-8-13.jpg"
} | 000847 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Redistribution of CLIC1 in T84 cells in response to forskolin. Confluent monolayers of T84 cells grown on filters were fixed directly (A) or pretreated with 10 μM forskolin in growth medium for 10 minutes prior to fixation (B). Cells were stained for CLIC1 and Z-section images created from 2 μm thick slices taken through stacks of confocal images taken at 0.2 μm intervals. Two representative Z-sections are shown from each specimen. The culture surface is at the bottom of each image, the apical surface of the cells at the top. Scale bar represents 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-2-1471-2121-8-8-9.jpg"
} | 000848 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "CLIC1 in T84 cells. Cells were grown to confluence on permeable supports, fixed, stained for CLIC1, and a stack of Z images at 0.2 μm intervals collected by confocal microscopy. A: image from the apex of the cells. 4μ: image taken 4 μm below image A. 8μ: image take 8 μm below image A. Z: vertical section generated from a 2 μm thick slice through the center of the stack of images. The scale bars represent 5 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-4-1471-2121-8-8-8.jpg"
} | 000849 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Colocalization of CLIC1 with markers of plasma membrane and nucleus following digitonin extraction. Panc1 cells were grown on glass coverslips and extracted with digitonin prior to PLP fixation and staining for CLIC1 (red) and subcellular compartment markers (green). The right column in each set is a merged image generated from the red (left column) and green (center column) channels. A, B. Two separate fields stained for CLIC1 (red) and Integrin α2 (green), a plasma membrane marker. C. Cells stained for CLIC1 (red) and Annexin2 (green), a plasma membrane marker. D. Cells stained for CLIC1 (red) and Nucleoporin p62 (green) a marker of nuclear envelope. The scale bar in panel A represents 20 μm and applies to all panels.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-5-1471-2121-8-8-6.jpg"
} | 000850 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Staining of mouse frozen tissue sections for CLIC1. Each row displays images from a single tissue. The left column contains sections stained with control antibody, the center column contains matched sections stained with AP1089. Scale bars displayed with the center image apply to both the left and center images of that row. The right column presents a high power view of the corresponding tissue stained with AP1089. A, B. Esophagus imaged with 4× objective. Bar = 200 μm. C. Esophagus with 20× objective showing basal epithelial cell layer. Bar = 50 μm. D, E. Glandular stomach with 10× objective. Bar = 100 μm. F. Surface epithelium of glandular stomach with 40× objective. G. Subepithelial glands of glandular stomach with 40× objective. Bar = 20 μm, applies to both panel F and G. H, I. Small intestine (jejunum) with 20× objective. Bar = 50 μm. J. Small intestine with 60× objective. Bar = 20 μm. K, L: Colon with 20× objective. Bar = 50 μm. M. Colon with 60× objective. Bar = 20 μm. N, O. Liver with 20× objective. Bar = 50 μm. P. Liver with 60× objective, bar = 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-7-1471-2121-8-8-2.jpg"
} | 000851 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "CLIC1 in MPTC cells. MPTC cell line was grown on collagen-coated filters, fixed, and stained with AP1089. Confocal images from the apical pole of the cell (A) and 5 microns (B) or 10 microns (C) below are shown. Scale bar represents 10 microns. D. Z section taken from a separate field along a single pixel line using 153 planes of focus covering 24 microns in depth. Scale bar represents 10 microns.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820597-9-1471-2121-8-8-12.jpg"
} | 000852 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Embryo Polarity Redirected by Ectopic CheFz1 or CheFz3(A) Differential interference contrast images of embryos following injection of 0.5 mg/ml RNA coding for CheFz1 or CheFz3 into eggs before fertilisation compared to uninjected controls. Stages as indicated.(B) Confocal images of early gastrula embryos stained with phalloidin/ToPro3 following morpholino injection into the egg (left column) and with polarity restored by injection of 0.5 mg/ml RNA (lacking the morpholino target site) into one blastomere at the two-cell stage (right two columns). Top row: CheFz1 misexpression; Bottom row: CheFz3 misexpression. Blue indicates the progeny of the RNA-injected cell revealed by fluorescent dextran. Arrows indicate ectopic pointed oral poles. The original position of the egg animal pole is deduced to lie on the injected–uninjected boundary (asterisk) since the first cleavage passes through the animal pole, and can be further pinpointed by residual endogenous polarity in CheFz1-injected embryos. Bars = 40 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820609-0-pbiop0050070pg007.jpg"
} | 000853 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Global β-Catenin Stabilisation Uncouples Oralisation from CheFz RNA Levels(A) Confocal images of phalloidin/ToPro3-stained early gastrula embryos (see Figure 2) showing characteristic phenotypes obtained following Wnt pathway activation by treatment with 50 mM LiCl from the two-cell stage (bottom row) compared with untreated controls (top row).(B) Representative in situ hybridisation images of control and LiCl-treated embryos fixed at the early gastrula stage, with probes as indicated (weak oral CheFz1 expression indicated by arrows in controls).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820609-3-pbiop0050070pg006.jpg"
} | 000854 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Reciprocal Regulation of CheFz1 and CheFz3Representative in situ hybridisation images obtained with CheFz1 and CheFz3 probes on control and morpholino-injected embryos. The arrow points to the weak oral CheFz1 expression domain in controls at this stage. Each morpholino causes massive up-regulation of the other RNA, albeit with vestiges of an oral–aboral gradient still discernable. A mixture of both morpholinos caused simultaneous up-regulation of both RNAs.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820609-4-pbiop0050070pg005.jpg"
} | 000855 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Opposing Localisation of CheFz1 and CheFz3 RNAs during Early DevelopmentIn situ hybridisation of C. hemisphaerica eggs, embryos, and planula larvae (fixed at 1 and 3 d after fertilisation), showing the concentration of CheFz1 RNA (top row) in the animal cytoplasm of the egg and around the nuclei during first cleavage, and its graded oral–aboral distribution in blastula and early gastrula stages. CheFz1RNA levels declined during gastrulation and subsequently remained low throughout all regions, with some increase in the oral endoderm during planula development. CheFz3 RNA (middle row) was localised strongly to the vegetal egg cortex, becoming concentrated in the vegetal part of the cleavage furrow during cytokinesis, and then to the presumptive aboral pole throughout embryonic and larval development. Asterisks mark nuclei in eggs. In medusa and polyp stages (bottom row), low levels of CheFz1 RNA were detected in all regions, including the tentacle bulb (tb), while CheFz3 RNA was detected strongly in specific regions of the endoderm: the circular canal (cc) and a juxta-oral band of the manubrium (arrows). All embryos and polyps are oriented with the oral pole pointing up. Bars = 40 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820609-5-pbiop0050070pg002.jpg"
} | 000856 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "CheFz1 and CheFz3 Regulate the Canonical Wnt Pathway and Direct Oral and Aboral-Specific Gene Expression(A) Activation of the canonical Wnt pathway in mid-blastula–stage control embryos (top row) and embryos derived from CheFz1-Mo (middle row)– or CheFz3-Mo (bottom row)–injected eggs, visualised by injecting eggs prior to fertilisation with RNA coding for a β-catenin–Venus fusion protein. Coinjected rhodamine dextran was distributed uniformly (not shown). β-catenin–Venus was stabilised specifically in the oral half of control embryos. CheFz1-Mo reduced β-catenin–Venus to barely detectable levels, while CheFz3-Mo caused it to accumulate in all cells.(B) Characteristic phenotypes of embryos derived from CheFz1-Mo– or CheFz3-Mo–injected eggs compared with uninjected controls. Differential interference contrast images of early gastrula-stage embryos: arrows indicate ingressing cells. CheFz1-Mo severely reduced the extent of cell ingression, although did not prevent oral thickening of the epidermal layer, while CheFz3-Mo caused expansion of the zone of cell ingression across most of the embryo.(C) Confocal images of similar early gastrula-stage embryos, with cell contours visualised using fluorescent phalloidin (green) and nuclei with ToPro3 (red).(D) Equivalent images of planula-stage embryos (1 d after fertilisation). There is a clear deficit in endoderm formation in embryos of CheFz1-Mo–injected but not in control CheFz1-5mp-Mo–injected embryos. CheFz3-Mo embryos show both endoderm and ectoderm, but oral–aboral polarity is disrupted.(E) Representative in situ hybridisation images of early gastrula embryos showing abolition of the oral CheBra expression territory following CheFz1-Mo injection, and expansion following CheFz3-Mo injection.(F) Aboral CheFoxQ2a expression at the same stage was abolished following CheFz3-Mo injection but expanded following CheFz3-Mo injection.In all panels, embryos are oriented with the oral pole up. Bars = 40 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820609-6-pbiop0050070pg003.jpg"
} | 000857 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Dynamic localization of Ins(1,4,5)P3 3-kinase A in postsynaptic spines. The figure shows IP3KA transfected into hippocampal neurones, and illustrates (A) before, (B) 2 min after, treatment with 100 μM glutamate, and (C) 20 min after removal of 100 μM glutamate and the addition of MK-801, an NMDA receptor antagonist. The reversible shift from the spines to the shaft induced by glutamate is plainly visible. See (Schell and Irvine, 2006) for all details.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820747-1-gr2.jpg"
} | 000858 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Part of intraoperative representative radiograph showing electrodes and pressure transducer inserted from the convex side of the scoliotic curve (same patient as figure 1).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820774-3-1748-7161-2-4-2.jpg"
} | 000859 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Photograph of intraoperative measurement set up. Rostral is to the left, caudal to the right. Three electrodes can be seen inserted through the convex annulus of three adjacent discs. The pressure transducer is seen inserted into the middle disc.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820774-7-1748-7161-2-4-1.jpg"
} | 000860 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Short term fate map of Math1-lacZ cells generated after conditional ablation of Notch1 in the cerebellar primordium. (a-d) Coronal sections from E14.5 floxNotch1 (a, c) and En1-cre; floxNotch1 (b, d) embryos stained for β-galactosidase activity. Long arrows indicate the approximate position of the MPT and LPB neurons, and short arrows indicate the position of the DCN. (e, f) Immunohistochemical staining for calbindin on adjacent sections to (a, b) shows an increase in the LPB neuron hindbrain population (white arrows). The asterisk in (e) indicates calbindin positive afferents that are absent in the mutants. Quantification of β-gal+ cells in the control and mutant brains across rostral to caudal tissue sections shows the decrease in DCN in the mutant. Scale bar in (f) represents 300 μm. CB, cerebellum; MB, midbrain; RL, rhombic lip.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-0-1749-8104-2-5-4.jpg"
} | 000861 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Conditional loss of Notch1 in the cerebellar primordium results in severe hypoplasia and loss of Purkinje cell precursors. (a, b) Sagittal whole mount view of E16.5 floxNotch1 (a) and En1-cre; floxNotch1 (b) brains. The arrows in (a, b) indicate the position of the cerebellum. (c-f) Sagittal cryosections of floxNotch1 (c, e) and En1-cre; floxNotch1 (d, f) cerebella stained by hematoxylin and eosin histochemistry (c, d) or by immunohistochemistry for calbindin (e, f). Boxes in (c, d) indicate the approximate photographic fields in (e, f). Dashed box in (f) delineates the residual cerebellum in the mutant embryo. Scale bars in (e, f) represent 300 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-1-1749-8104-2-5-3.jpg"
} | 000862 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Conditional loss of Notch1 in the early embryonic cerebellum results in precocious differentiation at the expense of the progenitor pool. (a) Whole mount β-galactosidase staining of an E9.5 En1cre; Rosa-stopLacZ reveals the extent of recombination across the mid-hindbrain region. (b-g) In situ hybridization with antisense probes for (b, c)Notch1, (d, e)Mash1, and (f, g) Math1 on sagittal cryosections of the cerebellar primordia from E10.5 floxNotch1 (b, d, f) and En1cre; floxNotch1 (c, e, g) embryos. (h, i) Immunohistochemistry for Math1 on E10.5 floxNotch1 (h) and En1cre; floxNotch1 (i) tissue cryosections. (j-o) In situ hybridization with antisense probes for (j, k)Notch1, (l, m) Mash1, and (n, o) Math1 on sagittal cryosections of the embryonic cerebella from E12.5 floxNotch1 (j, l, n) and Engrailed1-cre; floxNotch1 (k, m, o) embryos. The r1/r2 boundary is indicated by the arrow in (k). Brackets in (m, n) indicate the extent of Math1 induction in the VZ. (p, q) Immunohistochemistry for Math1 (white arrows) on E12.5 floxNotch1 (p) and En1cre; floxNotch1 (q) tissue cryosections. Scale bars in (g, o) represent 300 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-2-1749-8104-2-5-2.jpg"
} | 000863 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Notch1 activity regulates cell fate decisions in the embryonic cerebellum. Pseudotyped bicistronic retroviruses expressing PLAP and full length Delta1 or the Notch1 ICD were injected into the ventricle of E9.5 embryos in utero using ultrasound backscatter microscopy, and the pups sacrificed at P21. Brains of infected animals were cryosectioned sagittally and stained for alkaline phosphatase using NBT/BCIP histochemistry. (a, d) Control experiment with retrovirus expressing alkaline phosphatase alone. (b, e) Retroviral expression of Delta1 in E9.5 cerebellar progenitors yields granule cells at P21; the inset in (e) shows sections stained by immunohistochemistry for PLAP (red) and Zic2 (green). (c, f) Retroviral expression of the Notch1 ICD in E9.5 cerebellar progenitors results in Bergmann glia at P21; inset in (f) shows sections stained for PLAP (green) and BLBP (red). Scale bars in (c, f) represent 300 μm. ICD, intracellular domain; IGL, internal granule layer; ML, molecular layer.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-3-1749-8104-2-5-5.jpg"
} | 000864 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Interaction between Notch and BMP receptor signaling in the cerebellar primordium. (a-f) Stage 10 to 12 chick embryos were electroporated in ovo with a GFP reporter plasmid and the expression plasmids described below. After two days of incubation, embryos were processed for cryosectioning, and the sections processed by in situ hybridization for Cath1. Adjacent sections were photographed for GFP and Cath1 in each experiment. (a, b) GFP alone. (c, d) GFP and caBMPR1b. (e, f) GFP, Notch1 ICD, and caBMPR1b. In the case of caBMPR1b and Notch1 ICD/caBMPR1b electroporations, additional sections were stained by immunohistochemistry for GFP (green) and phosphorylated (g, i, j) Smad1 and (h, k, l) Msx1/2 expression (red; green and red channels are shown separately to the left of each overlay). The rostral cerebellar primordium is outlined with a dashed oval. In the caBMPR and Notch1 ICD/caBMPR Msx1/2 stainings shown in (k, l), GFP/Msx1/2+ and GFP/Msx1/2- cells were counted across sections from three electroporated cerebella each and represented as columns. Scale bar in (f) represents 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-4-1749-8104-2-5-6.jpg"
} | 000865 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Notch and BMP signaling components in the embryonic cerebellar primordium. Sagittal cryosections of E12.5 wild-type brains were stained by in situ hybridization with antisense probes for Notch1, Mash1, Hes5, Msx2, Math1 and Delta1. Differentiating neural precursors in the ventricular zone (VZ) and rhombic lip (RL) are delineated by Mash1 and Math1, respectively. Msx2 expression is indicative of BMP receptor signaling, and overlaps with the Math1+ territory at this stage. Hes5 and Delta1 are expressed throughout the cerebellar progenitor population, and are generally indicative of high and low Notch1 activity, respectively. The field shown in these panels corresponds to the box in the schematic (inset). Scale bar represents 300 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820780-5-1749-8104-2-5-1.jpg"
} | 000866 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Real-time B-mode high frequency USG findings of the recurrent lesion: An oval, hypoechoic solid lesion anteriosuperior to a scar in the subcutaneous region of the right upper inner thigh.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820783-0-1477-7819-5-28-1.jpg"
} | 000867 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Proximal-type epithelioid sarcoma of perineum. 2A. Tumor cells showing compact sheets of polygonal cells. Focal tumoral necrosis noted. H&E × 200. 2B. Sheets of large cells with occasional intracytoplasmic inclusions (inset) and little intervening stroma. H&E × 400. 2C. A 'pseudoangiosarcomatous' pattern. Tumor cells arranged in a disintegrating perivascular pattern. H&E × 400. 2D. Prominent multi nodular pattern of tumor. Reticulin silver stain × 100.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820783-1-1477-7819-5-28-2.jpg"
} | 000868 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "IHC results. 3A. Tumor cells displaying positive immunoreactivity for CK7. DAB × 400. 3B. Tumor cells displaying strong membranous positivity for EMA. DAB × 200. 3C. Tumor cells showing intra cytoplasmic dot-like expression for desmin. DAB × 400. 3D. Strong, diffuse, membranous positivity for CD34. DAB × 400. Positive internal control in the vascular endothelial cells noted.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820783-2-1477-7819-5-28-3.jpg"
} | 000869 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Contrast-enhanced axial orbital computed tomography showing a well-circumscirbed mass at lateral aspect of the left orbit without bone involvement.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820784-0-1477-7819-5-29-1.jpg"
} | 000870 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Hematoxylin-eosin staining (100× magnification) showing poorly differentiated adenocarcinoma, suggesting malignant carcinomatous changes in the recurrent pleomorphic adenoma. Nuclear atypia and prominent mitosis are noted.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820784-2-1477-7819-5-29-2.jpg"
} | 000871 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Showing the treatment plan of gamma knife radiosurgey.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820784-3-1477-7819-5-29-3.jpg"
} | 000872 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Follow-up brain MR images revealing a slightly decreased tumor size extending to orbital, cavernous sinus, infratemporal fossa, and clivus. However, small nodular enhancing lesion on the interhemispheric fissue and superior cerebellar peduncle, and leptomeningeal seeding is suspected.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820784-4-1477-7819-5-29-4.jpg"
} | 000873 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Description of the changes in foot alignment while lying supine in an unloaded position. The left panel shows a symmetrical position. The middle panel shows a slight displacement of the Y axis. The right panel shows a more pronounced displacement of the foot in the Y axis corresponding to inversion.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820789-1-1471-2474-8-17-6.jpg"
} | 000874 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "A TAO patient showing complex changes of facial symmetry. These include Y+ changes in the head on neck position, of the orbital axes as well as on the alignment of the eyes on the orbit.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820789-12-1471-2474-8-17-3.jpg"
} | 000875 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Metabolic imaging using 18F-Fluorodeoxyglucose (18F-FDG) showing metabolic activity in the lateral abdomen (dark areas). These areas are located around the Dai mai point which has been evaluated in our study. The accompanying CT images shows a clear deviation of the body axis at the level of the trunk. The images are reproduced with kind permission of the Society of Nuclear Medicine [166].",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820789-16-1471-2474-8-17-16.jpg"
} | 000876 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Image fusion of CT and scintigraphic data showing diffuse Octreotide uptake in the lower limbs of a patient with TAO. Octreotide uptake represents inflammatory activity. Taken from [49]. Each reconstruction plane is labeled in the upper left corner of the image. The right lower image is an anatomical reconstruction of the surface of the leg.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820789-4-1471-2474-8-17-12.jpg"
} | 000877 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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{
"caption": "Histological comparison of control CD-1 and LADY tumor. A: H&E staining of the CG from normal litter mate B: H&E staining of the CG from a LADY tumor at 16 weeks of age.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820793-3-1476-4598-6-19-1.jpg"
} | 000878 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Altered Differentiation of HCs to “PreHC-Like” CellsAnalyses of the proximal tibial growth plate of 10-day-old mice.(A–D and F) In situ hybridization using specific markers for resting, proliferating chondrocytes, and preHCs (Col2a1) (A), preHCs (Ppr) (B), preHCs and HCs (Ihh) (C), proliferating chondrocytes (Ptc) (D), and terminal HCs (Opn) (F). The chondro-osseous junction is depicted by a yellow line).(E) To show that altered differentiation took place after the initiation of hypertrophy, X-gal staining was performed on 10-d-old Col10a1-Cre/ROSA26 Cre reporter (R26R) mice with or without the 13del transgene. Positive staining appears as pink under dark field. The inset in the 13del panel shows co-localized X-gal staining (pink) and Ppr in situ hybridization signal (white dots) in mid-lower HZ of the triple mutant.(G) In situ hybridization for Opn and Ppr in consecutive sections. In some HCs of 13del mice, Opn and Ppr are co-expressed (yellow-circled cells). In wt mice, the expression profiles of Opn and Ppr are mutually exclusive (see red- and green-circled cells). Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-0-pbiop0050044pg005.jpg"
} | 000879 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Intracellular Accumulation of 13del Proteins(A) In situ hybridization for wt Col10a1 or 13del transcripts at 10-d-old proximal tibial growth plates.(B) Immunofluorescence of cryosections immunostained with a 13del-specific antibody (13del Ab, green fluorescence). Absence of staining in the HZ of wt littermates showed the antibody is specific for 13del protein (Figure 1G, wt panel). Red fluorescence signal marks concanavalin A (ConA) in the ER. Localization of 13del proteins within the ER (yellow) is shown by the overlapping green and red fluorescence signals. Insets show extracellular immunostaining for normal collagen X in wt and 13del mice.(C) Immunohistochemistry using 13del-specific antibody on paraffin-embedded proximal tibial growth plate sections. Specific staining (brown) was seen only in 13del HZ. The sections were not counterstained in order to access the intensity of staining more easily. Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-1-pbiop0050044pg002.jpg"
} | 000880 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Induction of ERSS in 13del HCsAnalyses of 10-d-old proximal tibial growth plates.(A) Electron micrographs of wt and 13del HCs showing distended and fragmented ER (bar indicates 1 μm).(B) RT-PCR of total RNA from hypertrophic cartilage: 469 bp: unspliced Xbp1 mRNA; 443 bp: spliced Xbp1s. Sequencing of the 443-bp PCR-fragment confirmed that it is the product of Xbp1s mRNA (unpublished data).(C–E) Expression of XBP1s and BiP revealed by in situ hybridization (D) and immunostaining (C and E).(F and G) Immunohistochemical detection of CHOP (F) and p53 (G). The boxes in (G) indicate the region of higher magnification shown in the bottom left corner.Color contrast of the low-power magnification images in (C–G) were adjusted as described in Materials and Methods. Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-2-pbiop0050044pg003.jpg"
} | 000881 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Abnormal Cell-Cycle Regulation in 13del HCs(A) BrdU pulse/chase assay. Left panel; 5-d-old mice were injected with a single dose of BrdU and sacrificed 2 h later. Right panel; 8-d-old mice received two pulses of BrdU with an intervening interval of 6 h and were sacrificed 48 h after the first injection. The rate of chondrocyte hypertrophy is similar in wt and 13del mice; the most distally located BrdU-labeled cells (arrowheads) occurred at the same level relative to the onset of the HZ.(B–D) Immunohistochemical detection of p57Kip2 (B), cyclin D1 (C), and PCNA (D). The boxes in (C and D) indicate the regions of higher magnification shown in the bottom left corner.Color contrast of the low-power magnification images in (A–D) were adjusted as described in Materials and Methods. Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-3-pbiop0050044pg004.jpg"
} | 000882 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The Dynamics of Transgene and BiP Expression, HZ Expansion, and Altered Differentiation during Prenatal GrowthGene expression analyses on the proximal tibial growth plates from 14.5 (A1–A5 and A1′–A5′), 15.5 (B1–B5 and B1′–B5′), and 17.5 dpc (C1–C5 and C1′–C5′) mice. In situ hybridization was performed on paraffin sections using probes detecting Col10a1 (first column in both 13del and wt sections), 13del (second column in each section), BiP (third column in each section), Ppr (fourth column in each section), and Opn (fifth column in each section). Brackets indicate HZ. Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-5-pbiop0050044pg007.jpg"
} | 000883 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The Signal for the Altered Differentiation of 13del HCs Is Cell AutonomousAnalyses of EGFP/wt and EGFP/13del chimeras.(A, B, and C) In EGFP/13del chimeras, normal (wt) cells express EGFP.(D, E, and F) 13del cells are identified by in situ hybridization with a 64-bp probe specific for the 13del transcripts. (D′) shows expression of Col10a1 in the normal EGFP/wt chimera (consecutive section).(G–I) Immunohistochemical detection for BiP.(J–L) Immunohistochemical detection for p57Kip2.(M–O) In situ hybridization for Ppr. The insets in (M) and (N) show regions in the LHZ containing wt and 13del cells, respectively, at higher magnification. (N) shows marked re-expression of Ppr in 13del cells.Chondro-osseous junctions are traced using a red or yellow line. Color contrast of (A–C) and (G–L) was adjusted as described in Materials and Methods. Bar indicates 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820825-8-pbiop0050044pg006.jpg"
} | 000884 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "N and p50-U1 Associate In Vivo(A) The BiFC assay was used to demonstrate the ability of N and p50-U1 to associate in living tissue. gN-YN (column 1) alone and p50-U1-YC (column 2) alone do not produce fluorescence in N. benthamiana tissue. Co-expression of gN-YN and p50-U1-YC produces Citrine fluorescence (column 3), demonstrating a close association between N and p50-U1. GUS-YC is used as control for the specificity of associations involving gN-YN (column 4). Citrine fluorescence was imaged with the 514-nm laser line of a 15-mW argon laser. Scale bar represents 20 μm.(B) p50-U1-Ob-YC expressed alone does not produce fluorescence (column 1). Co-expression of gN-YN and the non-eliciting p50-U1-Ob-YC does not produce Citrine fluorescence (column 2), indicating that they do not associate in vivo. Scale bar represents 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820829-2-pbiop0050068pg005.jpg"
} | 000885 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "N and p50-U1 Are Cytoplasmic and Nuclear(A) gN-Citrine and p50-U1-Cerulean alone do not cause HR cell death on N. benthamiana plants that do not contain N (left), whereas co-expression causes death (right).(B) Expression of gN-Citrine (lane 1) is confirmed by detection with anti-GFP antibodies. Lane 2 is an empty vector control. M is the size marker, and protein sizes are shown in kDa.(C) Expression of p50-U1-Cerulean (lane 1) and p50-U1-Ob-Cerulean (lane 2) is confirmed by Western blot with anti-GFP antibodies. Lane 3 is empty vector control. M is the size marker, and protein sizes are shown in kDa.(D) Localization of gN-Citrine by fluorescence microcopy. gN-Citrine is present in the cytoplasm and nuclei of cells (column 2). Citrine only (column 1) is shown for comparison. Structures in red are chloroplasts. The 514-nm laser line of a 15-mW argon laser and the 543-nm laser line of a 5-mW helium neon laser with appropriate emission filters were used to image Citrine and chloroplast autofluorescence, respectively. Scale bar represents 20 μm.(E) Localization of p50-Cerulean. p50-U1-Cerulean (column 2) is found in the cytoplasm and nuclei of transfected cells. Cerulean alone is shown for comparison (column 1). p50-Ob-Cerulean from a non-eliciting strain of TMV is chloroplastic (column 3), but a p50-U1-Ob-Cerulean chimera shows the same localization as p50-U1-Cerulean (column 4). The 458-nm laser line of a 15-mW argon laser and the 543-nm laser line of a 5-mW helium neon laser with appropriate emission filters were used to image Cerulean and chloroplast autofluorescence, respectively. Scale bars represent 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820829-3-pbiop0050068pg002.jpg"
} | 000886 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "N's TIR Domain Is Sufficient for Association with p50-U1(A) Co-immunoprecipitation of gN-TIR-TAP and p50-U1-Cerulean. N's TIR domain was expressed under the control of N's endogenous 5′ and 3′ regulatory regions. Extracts from tissue co-expressing N(TIR)-TAP (top panel, lanes 1 and 2) and p50-U1-Cerulean (middle panel, lane 1) or p50-U1-Ob-Cerulean (middle panel, lane 2) were incubated with anti-GFP antibodies. Immunoprecipitated complexes were separated by SDS-PAGE and probed with anti-MYC antibodies. N(TIR)-TAP was pulled down with p50-U1-Cerulean (bottom panel, lane 1), but not with p50-U1-Ob-Cerulean (bottom panel, lane 2). Lane M is the size marker, and protein sizes are shown in kDa.(B) BiFC between N(TIR)-YN and p50-U1-YC. N(TIR)-YN exhibits BiFC with p50-U1-YC (column 1), but not with p50-U1-Ob-YC (column 2). The TIR domains of two related R proteins, BS4 and RPP5, were tested for their ability to associate in vivo with p50-U1-YC. BS4(TIR)-YC and RPP5(TIR)-YC were co-expressed with p50-U1-YC, but were unable to exhibit BiFC (columns 3 and 4, respectively). Scale bar represents 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820829-4-pbiop0050068pg008.jpg"
} | 000887 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "N and p50 Are Found in the Soluble Fraction of Protein Extracts(A) gN-TAP or p50-U1-HA alone do not cause HR cell death in N. benthamiana plants that do not contain N (left), whereas co-expression causes death (right).(B) Western blot analysis was used to confirm expression of gN-TAP (lane 1). Lane 2 is an empty vector control. M is the size marker, and protein sizes are shown in kDa.(C) Western blot analysis was used to confirm expression of p50-U1-HA (lane 1) and p50-U1-Ob-HA (lane 2). Lane 3 is an empty vector control. M is the size marker, and protein sizes are shown in kDa.(D) Proteins extracts were centrifuged at 100,000×g to produce crude soluble (S100) and membrane (P100) fractions. Fractions were analyzed by Western blot analysis following separation by SDS-PAGE. gN-TAP is found in the soluble fraction in the absence (panel 1) and presence (panel 2) of TMV. PEPC is a cytoplasmic marker, and BiP is found in both the ER and cytoplasm.(E) p50-U1-HA (panel 1) and p50-U1-Ob-HA (panel 2) are both found in the soluble fraction.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820829-5-pbiop0050068pg001.jpg"
} | 000888 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The TIR Domain Is Critical for the Association of N and p50(A) NΔNB-YN and NΔLRR-YN produce Citrine fluorescence when co-expressed with p50-U1-YC (columns 1 and 3). The specificity of the associations was confirmed by co-expression with GUS-YC (columns 2 and 4).(B) NΔTIR-YN and p50-U1-YC do not exhibit BiFC when co-expressed (column 1). NΔTIR-YN also does not produce fluorescence with GUS-YC (column 2).(C) TIR domain point mutants that disrupt N-mediated resistance also do not show BiFC when co-expressed with p50-U1-YC (columns 1 and 3). As expected, they also do not produce fluorescence when co-expressed with GUS-YC (columns 2 and 4). Scale bar represents 20 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820829-7-pbiop0050068pg007.jpg"
} | 000889 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "TAF8 and SPT7L interact in vivo (A)The nuclear localization of TAF8 lacking the NLS [TAF8(1-294] depends on its in vivo interaction with SPT7L. HeLa cells were co-transfected with the indicated CFP- and YFP-containing expression vectors and localization of the expressed proteins were visualised by fluorescence microscopy. The images shown in each panel are representative of all the transfected cells. (B) Sensitized emission of YFP fusion proteins due to FRET was measured in two different experiments in the nucleus of 25 individual HeLa cells transfected with the indicated combinations of vectors expressing YFP and CFP fusion proteins. The mean value of FRET efficiency (in%) over the entire nucleus in each cell was calculated as described in the Materials and Methods. A threshold was set to 5%, above the highest value of the negative control CFP/YFP (see horizontal line in each graph), and for the other pairs only values above this level were averaged. The average value of the negative control is 1.03%. The average value for each pair is the following: CFP-TAF8/YFP-TAF10 = 16.9%; CFP-TAF8/YFP-SPT7L = 15.8%; CFP-TAF6/YFP-TAF9 = 27.1%. Note that the scale for the TAF6/TAF9 is different.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820849-2-ponep0000316pg003.jpg"
} | 000890 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Anaphase and telophase abnormalities in Rad21-depleted HeLa cells.(A–L) Cells fixed with Carnoy's and stained with Giemsa. (A–E) Control series showing the cytology of control-treated cells at different mitotic stages: (A,B) Metaphases - side and polar views; (C) Early anaphases – polar and side views; (E) Telophase. (F–L) Examples of cells treated with Rad21-specific siRNA: (F) Metaphase – polar view; (G–I) Aberrant anaphase – asynchronous anaphase, defined as described in Fig. 3; (J) Apolar telophase – separated sisters scattered in the cytoplasm (cell may be arrested in mitosis based on the level of chromosome condensation); (K–L) Telophases with chromosome bridges and lagging chromosomes; (M) Quantification of abnormal anaphase and telophase cells in control-treated and cohesin-depleted cells. At least 1000 cells were scored per sample.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820851-2-ponep0000318pg004.jpg"
} | 000891 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Rad21 depleted cells do not separate their sister chromatids in the presence of nocodazole.(A–E) HeLa cells synchronized in early S-phase and depleted of Rad21 (or mock depleted) with the indicated siRNA oligos as described in Figure 1, Protocol-B. Nocodazole was added after release from the S-phase synchrony and cells prepared for cytological analysis at the indicated time points. Cells with separated or partly separated sister chromatids were scored versus cells with cohered sisters, as depicted in C–E. For comparison, Sgo1-depleted cells were examined under the same conditions. Sgo1 depletion (∼80% depletion) was slightly less efficient that Rad21 depletion (data not shown). Note that the data in panel A are reproduced in panel B but with a different Y-axis scale. (F) HeLa cells were depleted of Rad21 (or mock depleted) with the indicated siRNA oligos as described in Figure 1, Protocol-A. Nocodazole was added 48 hours after siRNA transfection and cells prepared for cytological analysis at the indicated time points, then scored as in A–E (% separated sisters combines the categories shown in D+E).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820851-4-ponep0000318pg002.jpg"
} | 000892 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Cytological analysis of HeLa cells expressing non-cleavable (NC) Rad21.Control HeLa cells in which wild type MYC-Rad21 expression had been induced (A–E) were compared with HeLa in which NC-Rad21 (NC-MYC-Rad21) expression had been induced 72 hours prior to fixation and staining with Giemsa (F–O) or immuno-staining with CREST serum (P–T). Controls: (A,B) Normal cohesion and sister resolution in late prophase and early prometaphase; (C) Metaphase – polar view; (D) Anaphase -side view; (E) Telophase. NC-Rad21: (F–G) Delayed nucleolus disassembly in early prometaphase and delayed sister chromatid resolution (56% of prometaphases had the resolution defect); (H–I) Late prometaphase and metaphase cells with unresolved sisters (32%); (J) Metaphase – polar view; (K–L) Aberrant anaphases – centromere regions separating before arms (arrows) and some chromosomes segregating to the poles before other chromosomes have separated their sisters (28% were abnormal based on these criteria); (M) Cut telophase (26% of the telophases had this phenotype); (N) Apolar telophase (16% of the telophases had this phenotype); (O) Partially resolved diplochromosomes (5% of mitotics), in agreement with the described separase RNAi phenotype [41]; (P–T) DNA (DAPI; blue) and CREST (green) staining of kinetochores after NC-Rad21 induction. (P′–T′) DAPI channel only. (P) Metaphase; (Q) Early anaphase – one pair of kinetochores appears to be segregating prematurely; (R) Laggard kinetochores in anaphase; (S) Cut telophase - kinetochores have segregated to the poles despite deficient karyokinesis; (T) Bridged and laggard chromosomes in telophase.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1820851-5-ponep0000318pg005.jpg"
} | 000893 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Longitudinal sections of embryos after whole-mount in situ hybridisation, showing expression of: Cubilin at E6.5 (a) and E7.5 (b); Sfmbt2 at E6.5 (c) and E7.5 (d) (note that due to the distorted shape of the specimen this section does not fully pass through the lumen of the proamniotic cavity and the distal-most group of Sfmbt2-expressing cells in fact forms part of the extraembryonic ectoderm near the anterior amniotic fold); Ndrg1 at E6.5 (e) and E7.5 (f); Plet1 at E6.5 (g), E7.5 (h) (note also that the distal-most extraembryonic expression represents extraembryonic ectoderm near the anterior amniotic fold) and in the node at E8.0 (i); and Talia at E7.5 (j).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821012-4-1471-213X-7-8-3.jpg"
} | 000894 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Whole-mount in situ hybridisation of genes identified in microarray screen with differential expression patterns at stages as indicated. The two scale bars represent respectively 100 μm for all E5.5 and E6.5 images and 200 μm for all E7.5 and E8.0 images.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821012-7-1471-213X-7-8-2.jpg"
} | 000895 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The mitotic activity of the Wnt1-expressing basal layers is reduced. Samples described in the legend to Fig. 2 were immunostained for Ki67 (A), and the number of Ki67-positive cells per basal cell nucleus quantified (B).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821013-2-1471-213X-7-9-3.jpg"
} | 000896 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Wnt1 expression accelerates differentiation of organotypic cultures. NIK cells were transferred to culture conditions that promote epidermal stratification and development. Samples were taken at the timepoints indicated (12, 16 and 30 days), embedded, sectioned and stained with H&E (A) to reveal their z-axis morphology. The arrows indicate the pattern of growth typical of these cultures, relying on progenitor cells to feed the basal transit amplifying population that differentiate upwards into the stratified layers. Similar samples were processed for filaggrin-staining (B), and cell outlines were visualized with an antibody to E-cadherin.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821013-3-1471-213X-7-9-2.jpg"
} | 000897 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "The canonical Wnt signaling inhibitor, Dkk1, rescues Wnt-induced hyperkeratosis. NIK cultures were incubated with or without soluble dkk1 for 30 days, and the mitotic index of the basal layer of the organotypic cultures was assayed (A). The same cultures were stained with H&E, and cut on the z-axis to evaluate their differentiation (B).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821013-4-1471-213X-7-9-5.jpg"
} | 000898 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
|
{
"caption": "Age 13.3. After 22 months in braces and genioplasty. Left: Panoramic radiograph does not reveal root resorption. Teeth 27, 38, and 48 were removed at time of genioplasty. Right: Superimposition of lateral ceph before treatment with the fixed appliance (blue) (age 11.4) and after genioplasty (red) (age 13.3).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_106-PMC1821014-1-1746-160X-3-10-7.jpg"
} | 000899 | hf://datasets/vector-institute/open-pmc-18m@6109d453e9b8e2de3564869941b2e622faddd8d3/data_00000.tar |
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