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the role of industrial byproduct as supplementary cementitious material to partially replace cement has greatly contributed to sustainable environment metakaolin mk one of such byproduct is widely used to partial replacement of cement however during cement replacement at high percentage it may not be a good choice to improve the strength of concrete thus in the present study biocement a product of microbially induced carbonate precipitation is utilized in mkmodified cement mortars to improve its compressive strength despite of cement replacement with mk as high as the presented technology improved compressive strength of mortars by which was still comparable to those mortars with cement the results proved that biomineralization could be effectively used in reducing cement content without compromising compressive strength of mortars biocementation also reduced the porosity of mortars at all ages the process was characterized by semeds to observe bacteriallyinduced carbonate crystals and ftir spectroscopy to predict responsible bonding in the formation of calcium carbonate further xrd analysis identified biominerals formed in the mkmodified mortars the T0 also encourages combining biological role that cells can temporarily change their size in response to their surrounding environment and the stimuli they receive thus there must be a mechanism that normally keeps cell size constant while allowing a shift to an alternative size when necessary to investigate the molecular basis of mammalian cell size control we conducted a genetic screen in a human t cell L1 to identify genes involved in cell size regulation a prime candidate emerging from this screen increases cell size when it is overexpressed but reduces cell size when subjected to sirna knockdown several lines of evidence indicate that the product of this gene which we called largen regulates mrna translation in a manner associated with the upregulation of a TPS subset of mrnas many of which affect mitochondrial CF in fact cells overexpressing largen increase both mitochondrial mass and activity enhancing atp production these in vitro observations have been replicated in vivo using TGM models with a focus on these findings we discuss the possible contribution of mitochondria to the control of mammalian cell size | MEDAL |
dental burs are used extensively in dentistry to mechanically prepare tooth structures for restorations fillings yet little has been reported on the bur debris left behind in the teeth and whether it poses potential health risks to patients here it is aimed to image dental bur debris under dental fillings and allude to the potential health hazards that can be caused by this debris when left in direct contact with the biological surroundings specifically when the debris is made of a nonbiocompatible material nondestructive MCT using the biomedical imaging therapy facility id beamline at the canadian light source was pursued at kev and at a pixel size of µm to image dental bur fragments under a composite resin dental filling the burs cutting edges that produced the fragment were also chemically analyzed the technique revealed dental bur fragments of different sizes in different locations on the floor of the prepared surface of the teeth and under the filling which places them in direct contact with the dentinal tubules and the dentinal fluid circulating within them dispersive xray spectroscopy elemental analysis of the dental bur edges revealed that the fragments are made of tungsten carbidecobalt which is bioincompatible | MEDAL |
we demonstrate here the SP and flexibility of freesolution conjugate electrophoresis fsce as a method of separating dna fragments by electrophoresis with no sieving polymer network previous work introduced the coupling of fsce with ligase LDR ldr to detect point mutations even at low abundance compared to the wildtype dna here four large dragtags are used to achieve freesolution electrophoretic separation of ldr products ranging in size from to nt that correspond to mutations in the kras oncogene ldrfsce enabled electrophoretic resolution of these ldrfsce products by ce in min e vcm and by microchip electrophoresis in s e vcm the power of fsce is demonstrated in the unique characteristic of freesolution separations where the separation resolution is constant no matter the electric field strength by microchip electrophoresis the electric field was increased to the maximum of the SP supply e vcm and the ldrfsce products were separated in less than s with almost identical resolution to the separation at e vcm these results will aid the goal of screening kras mutations on integrated sampleinanswerout decedures by contrast malditof ms can identify bacterial species in a few minutes directly from a freshly grown colony various approaches have been developed for bacterial identification and the tau of this method has been demonstrated in several studies this technique should also be suitable for the classification of organisms to subspecies level and for the phenotypic PCD of certain antibiotic resistance mechanisms such as βlactamases production | MEDAL |
the SLO syndrome slos is a recessively inherited birth disorder caused by a defect in dehydrocholesterol betahydroxysteroid deltareductase the final enzyme in cholesterol biosynthesis to investigate in vivo regulation of the cholesterol biosynthetic pathway in slos we measured hepatic microsomal sterol concentrations and MICs of several key enzymes in the pathway including hmgcoa synthase hmgcoa reductase squalene synthase and dehydrocholesterol deltareductase in liver specimens from a patient with slos and controls hepatic microsomal dehydrocholesterol deltareductase activity in the patient was less than of the control mean and decreased cholesterol concentration and markedly increased and dehydrocholesterol concentrations were observed in the patients microsomes hmgcoa synthase and squalene synthase MICs in the patient were upregulated to and respectively while the activity of hmgcoa reductase the ratelimiting enzyme in the pathway was reduced to of the control mean downregulation of hmgcoa reductase activity in slos was supported by measuring plasma C2 of MVA the immediate product of hmgcoa reductase the levels in slos patients n were significantly low compared with agematched controls n vs nmoll p these results suggest that in most slos patients in vivo hmgcoa reductase is not stimulated in spite of blocked cholesterol biosynthetic pathway and reduced plasma and hepatic cholesterol concentrations | MEDAL |
myocardial performance was measured during maturation using isolated left ventricular papillary muscles from female wistar rats muscles were studied isometrically and isotonically stimulated at hz perfused with tyrode solution having an external calcium concentration of mm and maintained at degrees c heart and body weight increased rapidly during the first mo and then stabilized peak ISO developed tension time to peak IDT and time to half relaxation increased substantially during the to mo period with little NC in the peak rate of P0 rise and peak rate of P0 fall no difference in resting tension was noted among any groups forcevelocity curves from muscles from and moold animals show a significant agerelated decrease in the isotonic Vo by as much as between and mo of age thus maturation profoundly affects the contractile state of cardiac muscle with force of contraction increasing and velocity of shortening decreasing as a function of age in the rat | MEDAL |
continuous pumpdriven venovenous hemofiltration cvvh has become an established method for treatment of ARF arf since severe disturbances of micro circulation are intimately involved in the bad outcome of these patients the profile of endocrinological regulators of circulation was prospectively and serially measured in patients undergoing pumpdriven cvvh n patients with similar apache ii score but without arf and without cvvh were also studied endothelin et atrial natriuretic peptide anp vasopressin renin and catecholamine epinephrine norepinephrine plasma levels were measured before start of cvvh baseline in the noncvvh patients admission to intensive ECU and during the next days various hemodynamic parameters were additionally monitored map hr pap ci and right ventricular hemodynamics rvef rvedv rvesv remained almost unchanged in the cvvh patients and were without differences to the noncvvh group within the entire investigation period pcwp and rap were higher in the cvvh patients already at baseline rap mmhg pcwp mmhg p and remained elevated in the further course of the investigation renin plasma level was higher already at baseline in the cvvh patients pgml p and further increased during cvvh to pgml vasopressin increased only in the cvvh group from to pgml p abstract truncated at words | MEDAL |
guinea pigs infected with the xj prototype CS of junÃn virus reproduce the main features of AHF showing hemorrhages leukothrombocytopenia and focal lymphoid tissue necrosis viral lymphotropism is shown by the presence of viral antigens severe cytopathic effect and high virus titers in lymphoid organs a pronounced depression of humoral immune response to sheep erythrocytes as well as to the virus is described this T0 was carried out to determine whether cellular immune response was also modified and which cell populations were affected delayed hypersensitivity skin reaction to purified protein derivative was found to be markedly depressed T3 infection a noticeable decrease in both percentages and absolute t lymphocyte numbers detected by e rosettes in SP and CL nodes together with a low absolute t cell number in peripheral blood were observed total cell counts in spleen lymph NO and IP blood were also reduced on the contrary no modification in percentages of b lymphocytes as measured by eac rosettes was found these results indicate that cellmediated immunity is markedly impaired in guinea pigs infected with the xj CS of junÃn virus its relationship with the pathogenesis of the disease is discussed | MEDAL |
the present T0 examined the effect of season of the year on depression and other moods previous work primarily cross sectional or retrospective in design and involving clinically depressed or seasonally affective disordered samples has suggested that mood changes as a function of season however the literature also shows conflicting andor inconsistent findings about the extent and nature of this relationship importantly these prior studies have not adequately answered the question of whether there is a seasonal effect in nondepressed people the present T0 employed a longitudinal design and a large sample drawn from a normal population the results based on those participants for whom mood measures were collected in each season demonstrated strong seasonal effects beck depression inventory bdi scores were highest in winter and lowest in summer ratings on scales of hostility anger irritability and anxiety also showed very strong seasonal effects further analyses revealed that seasonal variation in bdi scores differed for females and males females had higher bdi scores that showed strong seasonal variation whereas males had lower bdi scores that did not vary significantly across season of the year | MEDAL |
biventricular noncompaction is a recently recognized rare form of cardiomyopathy it is characterized by altered structure of myocardial wall as a result of intrauterine arrest of compaction of the myocardial fibers in absence of coexisting congenital lesion LV is the most affected site for noncompaction but right VVI involvement has been reported in a few cases diagnosis is made with dimensional echocardiography or cardiac magnetic resonance imaging while major clinical manifestations are heart failure arrhythmias and embolic eventspulmonary artery hypertension pah has not been well elaborated in the literature we present a year old boy who had biventricular noncompaction complicated by severe pulmonary hypertension pulmonary hypertension may be a consequence of increased pulmonary venous pressures caused by systolic and diastolic left ventricular dysfunction secondary to noncompaction we review the literature particularly with reference topah in the context of our case | MEDAL |
BD has been established as a predictor of mortality and endpoint of resuscitation we hypothesized that in a significant subset of surgical intensive care patients BD is AA to hyperchloremic acidosis and that these patients experience lower mortality than those patients whose base deficits are AA to other causes seventyfive consecutive surgical intensive care patients with base deficits greater than were prospectively studied the etiology of the patients base deficits was determined by admission laboratory data patients were divided into those with hyperchloremic acidosis and those with acidosis from other causes mortality within these CG was compared by fishers exact test thirtyseven patients had hyperchloremic acidosis thirtythree patients had lactic acidosis three patients had base deficits AA to ketosis and two patients had base deficits AA to uremia there were no significant differences in age apache ii scores or volumes of resuscitation between the hyperchloremic group and the remaining patients there were four deaths in the hyperchloremic group and thirteen deaths in the remaining patients p hyperchloremic acidosis resulted from resuscitation with lactated ringers solution in of the hyperchloremic patients hyperchloremic acidosis is a common etiology of base deficit in the SICU it is associated with lower mortality than BD secondary to other causes moreover it is frequently induced following resuscitation with lactated ringers solution failure to properly diagnose this subset of acidotic patients may result in inappropriate clinical interventions due to the erroneous presumption of ongoing tissue hypoxia | MEDAL |
the gulf toadfish opsanus beta has a fully functional Orn urea cycle ouc that allows it to excrete nitrogenous waste in the form of urea interestingly urea is excreted in a pulse across the gill that lasts h and occurs once or twice a day both the AS hormone cortisol and the neurotransmitter serotonin ht are involved in the control of pulsatile urea excretion this and other evidence suggests that urea pulsing may be linked to toadfish social SMB the hypothesis of the present T0 was that toadfish urea pulses can be triggered by waterborne chemical cues from conspecifics our findings indicate that SE to seawater that held a donor conspecific for up to h preconditioned seawater pcsw induced a urea PP within h in naïve conspecifics compared to a pulse RL of h when exposed to seawater CT factors such as pcsw intensity and donor body mass influenced the PP RL response of naïve conspecifics fractionation and heat treatment of pcsw to narrow possible signal candidates revealed that the AS chemical was both watersoluble and HS fish exposed to urea cortisol or ht in seawater did not have a PP latency that was significantly different than seawater alone however ammonia perhaps in the form of nh | MEDAL |
neutrophils are the first cells arriving at CS of acute inflammation on their way from blood to the site of inflammation neutrophils have to adhere to EC cells ec to transverse the basement membrane and subsequently to travel through the interstitial matrix recently we have shown that human thy is an alternate ec receptor for the leukocyte integrin mac that contributes to leukocyte recruitment to sites of inflammation providing a new pathway for adhesion and transmigration of neutrophils here we studied the effect of thymediated adhesion on neutrophil functions IB of neutrophils to recombinant human thy stimulated the release of mmp from neutrophils resulting in their enhanced migration through collageniv and matrigel further we showed that neutrophil interaction with thy stimulated secretion of cxcl and thus could support the attraction of additional neutrophils to inflammatory sites blocking experiments confirmed the pivotal roles of thy on G1 dermal ec or fibroblasts and its counter receptor cd on neutrophils for the regulation of mmp and cxcl PR from neutrophils our results support the GA concept that the function of adhesion molecules in particular of human thy may not only be to provide mechanical support but also regulate neutrophil functions | MEDAL |
CSD of added cadmium in milk systems and the feasibility of removing Cd were investigated in milk containing ppm Cd of the added cadmium chloride was dispersed in the skim milk fraction and was associated with the cream fraction Cd was not bound strongly to any protein fraction the association of cadmium with acid casein whey proteins and the fat globule membrane was and of total added Cd with a min exposure thiosuccinylated aminoethyl cellulose thionitrocarboxyphenylated aminoethyl cellulose and reduced human hair removed and respectively of added Cd in SM previously equilibrated for h at c increasing equilibration time beyond h had no effect on RE whereas increasing ph decreased cadmium removal markedly | MEDAL |
the diazabicyclic CAA phosphonate dioxodiazabicyclononenylethylphosphonic acid was identified as a potent nmda antagonist it contains the alphaamino acid bioisostere diaminocyclobutenedione and an additional ring for conformational rigidity compound was as potent as cgs in the hcpp binding assay the stimulated htcp IB CA and the nmdainduced lethality MM in mice a single bolus dose of compound i.v. following permanent occlusion of middle cerebral artery mca in the rat reduced the size of infarcted tissue by structureactivity relationship sar studies have indicated that the six and eightmembered ring derivatives had diminished activity and that the twocarbon side chain length was optimum for nmda receptor affinity substitution on the ring was found to be counterproductive in the case of sterically demanding dimethyl CG and of no consequence in the case of an hbonding hydroxyl group replacement of the phosphonic acid group by either a carboxylic acid or a tetrazole group was unproductive the potent bicyclic nmda antagonists were synthesized efficiently by virture of their achiral nature and the ease of vinylgous NH formation from squaric acid esters compound being a unique nmda antagonist structural type with a favorable preclinical profile may offer advantages over existing nmda antagonists for the treatment of neurological disorders such as stroke and head trauma compound is currently under clinical evaluation as a neuroprotective agent for stroke | MEDAL |
ecological investigations of silkworms have revealed that eri silkworms samia cynthia ricini possess useful morphological and ecological characteristics for viruslike particle vlp production namely nonseasonal breeding longer lengths and heavier weights than bombyx mori silkworms furthermore when vector dna from bombyx mori nuclear polyhedrosis virus bmnpv which is unable to replicate in sf cells from eri silkworms was replaced with the autographa californica nuclear polyhedrosis virus acnpv vector three improved acnpv influenza virus recombinants capable of replication in sf cells were obtained although vlp antigens produced previously in silkworms were not evaluated individually the present recombinant fukushima fkh and anhui anh vlp antigens were detected in tissue fluids and fat bodies of eri silkworms here we aimed to determine the CF of the acnpv vector and p gene by expressing recombinants in sf cells and eri silkworm pupae the fkh recombinant produced high yields of haemagglutinin hapositive vlps showing a mean ha titre of million similarly high production of h ha vlps was observed in the fat bodies of eri silkworm pupae antigenic analysis and electron microscopy examination of erisilkwormproduced h ha vlps showed characteristic antigenicity and morphology similar to those of the influenza virus although fkh recombinants possessing the acnpv vector did not replicate in bmn cells the introduction of the helicase p gene from bmnpv resulted in their production in bmn and sf cells | MEDAL |
two L1 factors associated with skeletal fracture in older persons are intrinsic bone strength and risk of falling this T0 examined the role of timed up and go tug test performance a validated predictor of falling and hip areal bone mineral density bmd a validated predictor of bone strength in fracture prediction in a year longitudinal study | MEDAL |
movements of the foregut in crustaceans are produced by striated muscles that are innervated by motor neurons in the STG stg firing of the stg motor neurons generates excitatory junctional potentials ejps in the stomach muscles we now provide evidence for the existence of separate inhibitory and neuromodulatory innervations of some pyloric muscles in the foregut of several crabs callinectes sapidus cancer magister and cancer borealis electron microscopic examination of several pyloric muscles revealed three distinct types of nerve terminals excitatory terminals were readily identified by the spherical shape of their small clear SVs these terminals also housed a few large dense core vesicles inhibitory nerve terminals were recognized by the elliptical shape of their small clear synaptic LDV and contacted the muscles at welldefined synapses equipped with dense bar active zones bath application of gaba reduced the amplitudes of ejps in a pyloric muscle of c borealis consistent with the presence of gabaergic inhibitory innervation neuromodulatory terminals were characterized by their predominant population of large dense and DCVs these terminals formed synapses with presynaptic dense bars on the muscle as well as on the excitatory and inhibitory nerve terminals the presence of the inhibitory and neuromodulatory terminals creates a PET context for previously described reports of neuromodulatory actions on stomach muscles and suggests that the transfer CF from stg motor patterns to pyloric movement may be orchestrated by a complex innervation from sources outside of the stg itself | MEDAL |
this article discusses the experiences of DV among ethiopian refugees and immigrants in the united states a subset n of the larger T0 sample n participated in three focus groups with amharicspeaking survivors of domestic violence who were currently in or had left abusive relationships the research was conducted through a public health department university and community agency partnership findings show domestic violence as taking place within a context of immigration acculturation and rapid changes in family and social structure participants expressed a need for language and culturespecific DV support and advocacy as well as education programs regarding us laws and resources | MEDAL |
we investigated the properties of a galactosyltransferase galt that is involved in the synthesis of betagalactan side chains of pectins a membrane preparation of etiolated dayold soybean glycine max merr hypocotyls transferred cgal from udpcgal into intact and partially hydrolyzed lupin betagalactans of various chain lengths as exogenous acceptors while activity to endogenous acceptors was negligible Vmax occurred at ph and degrees c in the presence of mm mn and triton x the transfer reaction onto the unmodified commercial pectic galactan mr from lupin we used was very low but increased when the mr of the galactan was reduced by partial acid hydrolysis among the partially hydrolyzed galactans high mr average mr betagalactan was a more efficient acceptor specific activity pmol min mg protein than low mr average mr and polymers digestion of the radiolabeled product from high mr galactan with endobetagalactanase released mainly radioactive betagalactobiose and gal indicating that the transfer of cgal occurred through betalinkages hplc analysis showed that the enzyme also catalyzes incorporation of gal into pyridylaminated pa betagalactooligomers with degree of polymerization at least galpa chains were elongated by attachment of one two or three gal residues leading to the formation of galpa | MEDAL |
six day old rice seedlings oryza sativa l cv bahÃa were grown for or days in a nutrient solution with either cd mmoll or ni mmoll both cd and ni reduced the length of shoots and roots depending on the concentration and type of ion tested on the other hand the dry weight to fresh weight ratio was increased by heavy metal treatments especially in the aerial part of mmoll ni treated plants the application of mmoll cd and mmoll ni to the seedlings produced an inhibition of the transport of carbohydrate reserves from the seeds from which plants were developing to the rest of the plant Pn was also inhibited in treated plants however the total carbohydrate content in the shoots of these plants was higher than in controls thus the starch soluble sugars and sucrose content in the shoots of mmoll ni treated plants was respectively up to and times greater compared to controls the CSD of assimilates between organs was also affected by the treatment the carbohydrate content increased in the stem and second leaf but it was not affected or decreased in the root and third leaf although less evident ts ag asnlys cg and propro ct were investigated for association with overweight and obesity by a case control design overweight or obese subjects and lean NCs were recruited in harbin region in north china all three polymorphisms were genotyped by sequenom single nucleotide polymorphism snp detection system in both cases and controls genotypes for all three polymorphisms were in hardyweinberg equilibrium in control subjects both CG had similar CSD of alleles and genotypes created by the three coding polymorphisms of lepr gene no differences in frequencies of genotypes or alleles between cases and controls for any polymorphism individually were found by Ï analysis p p and p respectively further when the haplotypes of three polymorphisms were assessed no association for any haplotype of three polymorphisms was revealed in the present T0 the three coding polymorphisms in lepr gene were firstly investigated in a population of northern chinese it was suggested that the three coding polymorphisms in lepr gene were unlikely to have major effects on susceptibility to obesity in RNA chinese | MEDAL |
we studied the predictive impact of family satisfaction marital status and functional impairment on the trajectories of life satisfaction over the first years following medical treatment for traumatic spinal cord injury burns or interarticular fractures total n it was anticipated that fewer PET impairments being married and greater family satisfaction would predict higher life satisfaction trajectories | MEDAL |
the distribution of the androgen receptor ar in the adult rat testis was determined by biotinstreptavidin immunoperoxidase employing tissue embedded in polyester wax which preserves antigenicity without compromising tissue preservation the antibody probe used which has been characterized previously was an affinity purified rb polyclonal antibody raised to the amino terminus peptide of the rat ar within the interstitial compartment ar immunostaining was detected in some leydig cells and all smooth muscle cells forming the walls of blood vessels but EC cells of blood vessels were negative furthermore in those leydig cells that were clearly identified as exhibiting ar IS the intensity of the reaction varied in the ST ar immunostaining was observed in all peritubular myoid cell nuclei but not in the distal SL of lymphatic endothelial cells in sertoli cells nuclear ar immunostaining was stage TPS moderate ar immunostaining first became evident at late stage iv or early stage v of the cycle reached a robust peak at stages viiviii and then disappeared CR specific overnight SE to morphine at degrees cthe OD of tolerance to morphine unlike dependence appears to be a temperaturedependent process this also raises the possibility that naloxone possesses intrinsic negative agonism at morphine sensitive receptors which is manifested as a functional response only T3 adaptive changes in the MP following SE to morphine | MEDAL |
cyclooxygenase cox is highly expressed in tumor cells and has been regarded as a hallmarker for cancers but the excise regulatory mechanism of cox in tumorigenesis remains largely unknown here we pulled down and identified a novel cox regulator heterogeneous nuclear ribonucleoprotein ab hnrnpab which could specifically bind to cox core promoter and regulate RT growth in nonsmallcell lung cancers nsclcs knockdown of hnrnpab by shrna or sirna downregulated cox expression and prostaglandin e pge production and suppressed RT cell growth in nsclc cells in vitro and in vivo conversely overexpression of hnrnpab upregulated the C2 of cox and pge and promoted tumor cell growth we also showed that hnrnpab expression was positively correlated with cox expression in nsclc cell lines and tumor tissues and the upregulated expression of hnrnpab and cox predicted worse prognosis in nsclc patients furthermore we demonstrated that the activation of cox expression by hnrnpab was mediated through the cooperation with p a transcriptional coactivator in nsclc cells the hnrnpab could interact with p directly and be acetylated by p exogenous overexpression of p but not its histone AT hat domain deletion mutation augmented the acetylation of hnrnpab and enhanced its binding on cox promoter thereby promoted cox expression and lung cancer cell growth collectively our results demonstrate that hnrnpab promotes tumor cell growth by activating cox signaling in nsclc cells and imply that the hnrnpabcox pathway may be a potential therapeutic target for human lung cancers | MEDAL |
a simplified sulphidesilver technique for visualizing transitional metals in rat brain is described it consists of sulpzymin was also found to induce pptdinsp hydrolysis although the ionophore a was without effect both carbamylcholine and glucose induced an increase in pphosphatidic acid the results provide two independent pieces of evidence suggesting that phospholipase cmediated hydrolysis of polyphosphoinositides occurs as an early response in rat islets to either nutrient or neurotransmitter secretagogues | MEDAL |
abstract objective this study investigates the relationship between treatment regimen symptom severity comorbidities and health outcomes of paediatric patients with attentiondeficithyperactivity disorder adhd in central and eastern europe cee methods males and females aged years with adhd symptoms participated in this month prospective observational nonrandomised study PS and comorbidities were assessed using the child and adolescent symptom inventory parent checklists csi asi categories lo and the clinical global impressionsadhdseverity scale cgiadhds baseline data are presented results the T0 included patients from czech republic hungary romania slovakia and turkey psychiatrists made all diagnoses using the american psychiatric association DSM of mental disorders fourth edition text revision dsmiv world health organization international statistical classification of diseases and related health problems th revision icd and other criteria and respectively patients were grouped into two cohorts based on whether they were prescribed psycho andor pharmacotherapy n or not n patients receiving prescribed treatment were older and demonstrated higher symptom severity scores than those receiving no or other treatment most patients were prescribed CT for adhd at baseline conclusions continued assessment of this population may aid the treatment and outcomes of adhd in cee | MEDAL |
the gene pth encoding peptidyltrna hydrolase pth is essential for protein synthesis and viability of escherichia coli two pth mutants have been studied in depth a pthts mutant isolated as temperature CS and a pthrap mutant selected as nonpermissive for bacteriophage lambda vegetative growth here we show that each mutant protein is defective in a different way the pthts protein was very unstable in vivo both at degrees c and at permissive temperatures but its specific activity was comparable to that of the wildtype enzyme pthwt conversely the mutant pthrap protein had the same stability as pthwt but its TPS activity was low the thermosensitivity of the pthts mutant presumably ensues after pthts protein levels are reduced at degrees c conditions that increased the cellular pthts concentration a rise in gene copy number or diminished PD allowed cell growth at a nonpermissive temperature antibioticmediated inhibition of mrna and protein synthesis but not of peptidyltrna dropoff reduced pthts cell viability even at a permissive temperature based on these results we suggest that pthts protein being unstable in vivo supports cell viability only if its concentration is maintained above a threshold that allows general protein synthesis | MEDAL |
ventricular arrhythmia is one of the most important causes of death in industrialized countries and often accompanies myocardial infarction and heart failure in recent years modification of gapjunctional coupling has been proposed as a new antiarrhythmic principle we wanted to examine whether the gap junction modulator antiarrhythmic peptide aap exerts effects on human cardiac gap junctions whether the effect might be enhanced in uncoupled cells whether it affects electrical and metabolic coupling and which of the cardiac connexin isoforms cx cx cx may be affected | MEDAL |
pharmacological depolarization by kcl or veratrine reduced hdiprenorphine binding to opioid receptors in the hippocampal slice in a transient calciumdependent and peptidesensitive manner these results suggest that endogenous opioid MMP were released from synaptic terminals and competitively displaced hdiprenorphine IB to or hdiprenorphine binding was significantly reduced by calciumdependent depolarization throughout the hippocampus as determined by subsequent receptor autoradiography and quantitative densitometry displacement of binding was evident at CS in the ca and ca regions the dentate gyrus and the subiculum the most dramatic reduction was evident in stratum lacunosum moleculare of ca correlating the sites of maximal hdiprenorphine displacement with the previously described distribution of the opioid MMP suggests that the perforant path SF release enkephalins in stratum lacunosum moleculare of ca and stratum moleculare of the dentate gyrus and that MFs may release both dynorphins and enkephalins near SP of ca and stratum granulosum the lack of complete overlap between the CSD of opioid terminals and the sites of MSD indicates that these peptides may diffuse a moderate distance to their CS of action radioligand MSD defines the sites of endogenous opioid IB suggests the likely sources of peptide release and thus predicts the CS of endogenous opioid action within the hippocampus | MEDAL |
an inherited deficiency of adenosine deaminase ado deaminase adenosine aminohydrolase ec causes severe combined immunodeficiency disease in humans a similar deficiency in PNPase puo phosphorylase purinenucleosideorthophosphate ribosyltransferase ec engenders a selective cellular immune deficit to elucidate the possible metabolic basis for the contrasting immunologic phenotypes we compared the toxicity toward mature resting human lymphocytes of the ado deaminase substrates deoxyadenosine and adenosine and the puo phosphorylase ATP deoxyguanosine when ado deaminase was inhibited micromolar concentrations of deoxyadenosine progressively killed nondividing helper and CD8 TA but not B1 the toxicity required phosphorylation with subsequent datp formation the deoxyadenosine analogs chlorodeoxyadenosine fluorodeoxyadenosine and adenine arabinonucleoside also killed resting t cells cell death was unrelated to inhibition of adenosylhomocysteinase ec but was preceded by a gradual decline in atp levels as much as mm deoxyguanosine did not impair resting lymphocyte viability despite the synthesis of dgtp the combination of microm adenosine plus microm homocysteine thiolactone killed dividing lymphocytes but had no discernible toxic effect toward resting TA which accumulated adenosylhomocysteine over a hr period but thereafter excreted the nucleoside into the culture medium the different clinical syndromes associated with genetic deficiencies of ado deaminase and puo phosphorylase may be explained by the ability of datp to kill mature resting t lymphocytes by depleting atp C2 | MEDAL |
using the FO technique we tracked airway resistance continuously during QB qb and deep inspiration di thus observing fluctuations in resistance that may reflect mechanisms of airway stretch and renarrowing after di however the resistance may be depressed for a period not related to volume changes we hypothesized that this gradual increase in resistance T3 diinduced dilation was determined by a simple time constant furthermore to the extent that this effect reflects dynamic characteristics of airway renarrowing the resistance change after each tidal inspiration should also be constrained by this temporal limit a MM relating resistance fluctuations to the PLB pattern including both instantaneous and delayed effects was developed and applied to data from nonasthmatic and asthmatic subjects forced expiratory volume in s and respectively means sd after methacholine challenge dose and micromol respectively that resulted in respective forced expiratory volume in s reductions of and from baseline resistance was measured continuously for min of qb a di followed by a further minute of qb resistance values at end expiration ree and end inspiration were calculated we found that the CS of ree after di was best modeled by a powerlaw function of time rather than an exponential decay r compared with p furthermore the coefficient characterizing this renarrowing CF was close to equal to the coefficient characterizing the equivalent function of resistance change between each resistance value at end inpiration and subsequent ree during qb particularly in the nonasthmatic subjects for whom the intraclass rho was this suggests that the same timedependent AF determine renarrowing T3 both C1 and small breaths | MEDAL |
puromycinsensitive and insensitive aminopeptidase APM MICs are measured in several subcellular fractions of the rat brain cortex and subcortex during the first postnatal month tyrbetanaphthylamide has been used as substrate and microm puromycin as selective inhibitor we have found that puromycinsensitive aminopeptidase activity increases twofold in the synaptosomal and mitochondrial fractions in the first postnatal days just during the period of axonal and dendritic growth this enzyme also has significant agerelated changes in the nuclear fraction the developmental DP is different depending on the subcellular fraction analyzed significant developmental changes of puromycininsensitive aminopeptidase APM are only found in the myelinic and microsomal fractions and they are less significant than those found in the puromycinsensitive aminopeptidase it has been suggested that these enzyme activities could be involved in processes of cell proliferation differentiation and maturation | MEDAL |
the dominant evolutionary theory of physical attraction posits that attractiveness reflects physiological health and attraction is a mechanism for identifying a healthy mate previous studies have found that perceptions of the healthiest body mass index weight scaled for height bmi for women are close to healthy bmi guidelines while the most attractive bmi is significantly lower possibly pointing to an influence of sociocultural factors in determining attractive bmi however less is known about ideal body size for men further research has not addressed the role of body fat and muscle which have distinct relationships with health and are conflated in bmi in determining perceived health and attractiveness here we hypothesised that if attractiveness reflects physiological health the most attractive and healthy appearing BC should be in line with physiologically healthy BC thirty female and male observers were instructed to manipulate female and male body images in terms of their fat and muscle to optimise perceived health and separately attractiveness observers were unaware that they were manipulating the muscle and fat content of bodies the most attractive apparent fat mass for female HB was significantly lower than the healthiest appearing fat mass and was lower than the physiologically healthy range with no significant difference for muscle mass the optimal fat and muscle mass for mens bodies was in L1 with the healthy range male observers preferred a significantly lower overall male body mass than did female observers while the body fat and muscle associated with healthy and attractive appearance is broadly in line with physiologically healthy values deviations from this pattern suggest that future research should examine a possible role for internalization of body ideals in influencing perceptions of attractive BC particularly in women | MEDAL |
two weeks of FF soy MMP containing collagen peptides increased the levels of type i and iii tropocollagen and their mrnas in contrast the diet did not increase the mrna C2 of rat hyaluronan synthases serine palmitoyltransferase the ratelimiting enzyme of ceramide synthesis and HMG coenzyme a hmgcoa reductase the key enzyme of cholesterol synthesis these results suggest that FF of soy peptides with collagen peptides specifically enhanced the tropocollagen level in the skin | MEDAL |
the correct trial demonstrated survival benefits with regorafenib monotherapy in patients with treatmentrefractory M1 CC mcrc however the trials stringent eligibility criteria for regorafenib may limit its external validity we aimed to examine treatment attrition rates and eligibility for regorafenib in routine practice we identified patients at the BCCA diagnosed with mcrc who demonstrated disease progression or intolerable toxicity on or more lines of SVR therapy during the T0 timeframe panitumumab and cetuximab were only used in the chemorefractory setting data on clinicopathologic variables and patient outcomes were ascertained and analyzed eligibility was determined using the correct trial criteria a total of patients were identified among whom only were eligible for regorafenib in the panitumumab group and in the cetuximab group the main reasons for ineligibility in all patients were eastern cooperative oncology group performance status ecog ps an elevated total bilirubin and thromboembolic events in the past months no difference in eligibility for regorafenib was observed between patients previously receiving panitumumab or cetuximab p ci KM analyses showed that regorafenibeligible compared to regorafenibineligible patients had an increased median overall survival of versus months respectively p however cox proportional hazard analyses showed that only ecog ps rather than trial eligibility was correlated with outcomes the strict eligibility criteria disqualify most patients with treatmentrefractory mcrc for regorafenib therapy future trials should broaden the eligibility criteria to improve external validity | MEDAL |
in this work strategies for the robust localization of parallel resonant circuits are investigated these strategies are based on the subtraction of two images which ideally differ in signal intensity at the positions of the devices only to modulate their signal amplification and thereby generate the local variations the parallel resonant circuits are alternately detuned and retuned during the acquisition the integration of photodiodes into the devices permits their fast optical switching radial and spiral imaging sequences are modified to provide the data for the two images in addition to those for a conventional image in the same acquisition time the strategies were evaluated by phantom expittle is reported describing the nature of the antimicrobial compounds or their potential presence in commercial annatto colorant preparations this T0 was conducted to determine whether commonly available annatto extracts are capable of influencing the outgrowth of selected LA spoilage and pathogenic microorganisms disk diffusion and tube macrodilution techniques were used to determine the mics and mbcs of doublestrength watersoluble annatto extracts standard antibiotic disks were used as controls for the DD CA the results demonstrate that annatto has an GABA effect on bacillus cereus CP and staphylococcus aureus with mics of and volvol and diameters of inhibition of to to and to mm respectively a concentration of volvol inhibited the growth of streptococcus thermophilus LC subsp casei lactococcus lactis and paenibacillus polymyxa the mics for LM and enterococcus durans were and volvol respectively no activity was detected against lactobacillus plantarum bifidobacterium bifidum yeasts or selected gramnegative bacteria | MEDAL |
while increased uPA upa expression in breast cancer tissue is directly associated with poor prognosis recent evidence suggests that upa overexpression may suppress RT growth and prolong survival celecoxib has been shown to have antiangiogenic and antiproliferative properties we sought to determine if upa pa inhibitor pai and prostaglandin pge expression in nipple aspirate fluid naf and upa and pge expression in plasma were altered by celecoxib dose and concentration in women at increased breast CA risk | MEDAL |
plants are continually exposed to numerous environmental stresses to decrease damage caused by these potentially detrimental AF various stressrelated signaling cascades are G1 in plants one such stressresponsive signaling pathway the mitogenactivated protein kinase mapk module plays a critical role in diverse plant stress responses here we functionally characterized biotic and abiotic stressresponsive mapk genes ibmpk and ibmpk from sweetpotato ibmpk contain totally mapk conserved subdomains and the phosphorylating motif tey bacterially expressed ibmpk could be autophosphorylated in vitro and these proteins phosphorylated universal kinase substrate such as myelin basic protein ibmpk transcripts were expressed in leaf stem and root of sweetpotato cultivars with storage roots of various colors ibmpk and ibmpk were induced by various bioticabiotic AS treatments furthermore the kinase activity of ibmpk was induced during early nacl sa h | MEDAL |
since the descriptions of myoblastomas later denominated granular cell tumours many immunohistological and ultrastructural studies have been undertaken with the aim of establishing their histogenesis now the hypothesis of a neurogenic origin appears clear from the C1 number of cases diagnosed in geneva over a period of years we decided to do a retrospective study of these tumours with regard to their immunohistological phenotypes using the following markers s protein s neuron specific enolase nse vimentin CG glial fibrillary acidic protein gfap keratin epithelial membrane antigen ema desmin smooth muscle alpha actin of tumours were positive for s and for nse which confirms the neurogenic origin were vimentin positive with however a variable intensity all the other markers were negative immunohistological staining for s and nse may be useful tool in the diagnosis of these tumours | MEDAL |
two cases are reported of generalized pustular psoriasis compliocess of the psychotherapy | MEDAL |
severe traumatic HI in the elderly has been associated with poor outcomes however there is currently no consensus to direct management in these patients this study outlines the demographics injury characteristics management and outcome of the elderly trauma patients with severe head injury across a defined population | MEDAL |
multidimensional nmr spectra have traditionally been processed with the fast fourier transformation fft the availability of high field instruments the complexity of spectra of large proteins the narrow signal dispersion of some unstructured proteins and the time needed to record the necessary increments in the indirect dimensions to exploit the resolution of the highfield instruments make this traditional RPA unsatisfactory new procedures need to be developed beyond uniform sampling of the indirect dimensions and reconstruction methods other than the straight fft are necessary here we discuss approaches of nonuniform sampling nus and suitable reconstruction methods we expect that such methods will become standard for multidimensional nmr data acquisition with complex biological macromolecules and will dramatically enhance the power of modern biological nmr | MEDAL |
small bowel damage is frequent but underdiagnosed among critically ill patients with shock high catecholamine doses may have a deleterious effect on mesenteric blood flow plasma intestinal fatty acidbinding protein ifabp concentration is a marker of enterocyte damage whereas plasma citrulline concentration is a marker of functional enterocyte mass we hypothesized that high doses of catecholamines in critically ill patients may be associated with enterocyte damage this study aimed to determine the link between catecholamine use and dose with enterocyte damage this is a prospective observational T0 performed in a large regional university teaching hospital critically ill patients requiring EN andor norepinephrine at admission to a MICU icu were included as well as controls not receiving catecholamines we evaluated at admission plasma ifabp and citrulline concentrations ABD perfusion pressure app and variables relating to prognosis and treatment patients were categorized according to the quartiles of catecholamine dose at icu admission sixty critically ill patients receiving catecholamines and not receiving CAs were included plasma ifabp was higher among patients receiving catecholamine than in controls among patients receiving catecholamines a dose of γ kg min or more at icu admission was associated with a higher ifabp concentration a sepsisrelated organ failure assessment score higher than and plasma ifabp more than pg ml at icu admission were independently associated with day mortality odds ratio and odds ratio respectively catecholamine use is associated with ifabp elevation in critically ill patients critically ill patients receiving more than γ kg min of epinephrine andor norepinephrine at icu admission have high ifabp concentrations this suggests that enterocyte damage reflects the severity of shock and an adverse effect of CAs per se is possible | MEDAL |
the present review discusses the structure of the anticholinesterase organophosphates ops which are used predominantly as insecticides op poisoning can occur in a variety of situations and can be accidental or suicidal it is common in developing countries the cholinergic syndrome is caused by acetylcholinesterase inhibition and diagnosis is based on the clinical signs and PS as well as the measurement of inhibition of erythrocyte acetylcholinesterase andor plasma cholinesterase activity antidotal treatment is with atropine an enzyme reactivator such as pralidoxime and diazepam anticholinesterase ops may produce effects other than the acute ACh syndrome including the intermediate syndrome later effects may include organophosphorusinduced delayed neuropathy certain ops are exploited for their anticholinesterase effects including defoliants such as def herbicides such as glyphosate fire retardants and industrial intermediates the toxicology of this group is heterogeneous and they may or may not possess anticholinesterase activity | MEDAL |
twentyseven prostatic adenocarcinomas most of them well differentiated were examined for acid mucoproteins by the kreyberg and alcian blueperiodic acidschiff methods seventeen psure of rats to light can shift the phase of this enzymes CR light also rapidly reduces nocturnal enzyme activity intraventricular i.v. of carbachol a cholinergic agonist can mimic both of these effects CS and carbachol presumably act on the SCN these experiments demonstrate the feasibility of using a neuropharmacologic RPA to the mechanisms underlying rat circadian rhythms | MEDAL |
recent studies support a role for excitotoxicity in the OD of retinal ganglion cell rgc damage in subjects suffering from glaucoma coenzyme q coq an essential cofactor of the electron transport chain has been reported to afford neuroprotection preventing the formation of the mitochondrial permeability transition pore using an established animal MM of retinal ischemiareperfusion here we show that synaptic glutamate increases at min from beginning of reperfusion and delayed apoptosis in the rgc SL is seen at h intraocular administration of coq minimizes glu increase and affords neuroprotection suggesting that oxidative stress and SE failure might be implicated in the mechanisms of rgc death | MEDAL |
the present T0 was performed to elucidate the relationship between ctlacd molecules and stimulating tsab and blocking tbab antibodies to the tshreceptor tshr in graves disease cd and cd ctla are glycoprotein molecules which provide a potent costimulatory signal for tcell activation and proliferation via interactions with their ligands bb molecules which are present on the surface of antigenpresenting cells the aim of the study was to estimate the expression of cytotoxic tlymphocyteassociated antigen ctla cd cd b cd and b cd molecules on peripheral blood cells in patients with graves disease gd n mean age years and nontoxic nodular goiter ntng n mean age years in comparison with sex and agematched healthy control subjects n mean age years the expression of the costimulatory molecules on MNC cells was analyzed by threecolor FC using a coulter epics xl cytometer detection of tsab and tbab to the tshr using jpo cho cells in UF SS was measured by a highly sensitive commercial radioimmunoassay when compared with healthy control subjects and euthyroid patients with gd UT patients with gd showed a significant increase of cd p p and cd p ns t lymphocytes respectively after months of methimazole therapy the percentage of these cells in the IP blood of hyperthyroid patients returned to normal values in addition patients with gd showed an increase in the percentage of both b and b molecules on activated monocytes compared to patients with ntng p p respectively and healthy control subjects p p respectively in patients with UT gd there was a statistically significant positive correlation between the expression of ctla on the surface of PBT and the index of tsab antibodies r p as well as a negative correlation with tbab antibody titer r p however no such correlations were noted with regard to cd and antitpo antitg and trab antibodies we conclude that changes in the expression of costimulatory molecules on the surface of IP blood TA and their significant relationship with the level of antithyroid antibodies indicate an involvement of these molecules in the pathogenesis of gd | MEDAL |
eight hundred sixtysix patients with acute myocardial infarction ami were enrolled in a prospective T0 to determine optimal predictors of longterm prognosis during month mean followup there were cardiac deaths and nonfatal repeat amis twentynine variables from the history physical examination serum chemistries ambulatory monitor radionuclide ventriculogram and exercise test were arranged into in sequential CG according to the time at which results became available during hospitalization multivariate analysis LR and receiveroperator characteristic curves were used to assess improvement in prediction of mortality or repeat ami by addition of each group of variables the first group of independent predictors included rales left BBB and symptom status at month before admission addition of information from AM or SS chemistry did not improve prediction radionuclide ejection fraction made a statistically significant independent contribution to mortality prediction of the final group the only exercise test VL that contributed independently to prediction was whether the patients took the test however receiveroperator characteristic curves showed that improvement in sensitivity and specificity by addition of information from the RN scan and exercise test was clinically insignificant our results imply that costly tests T3 ami should be reserved for specific indications and not applied universally for prognosis although these tests were highly predictive individually each test generally added little to preexisting prognostic information | MEDAL |
HR caused by drugs andor their solvents in clinical conditions is a well documented observation but the mechanism of this reaction is poorly understood hence in this study the histamine releasing ability of cremophor e and six derivatives of hydroxystearic acid hsa were compared in two models the in vivo anaesthetized dog and the in vitro isolated rat PMC the results obtained in both systems differed markedly only one compound dh the diester of hsa with polyethylene glycol released histamine in both systems the two substances which exhibited the weakest histamine releasing ability in the dog MM almost inactive at the doses given were powerful releasers of histamine from rat peritoneal mast cells tn hsa polymerized with DDE oxide and me the monoester of hsa esterified with PE glycol the release of histamine from rat peritoneal mast cells was potentiated as the temperature was elevated above degrees c due to the heterogeneity of mast cells from both different species and different tissues in the same animal it is important to choose the appropriate predictive model for clinically important adverse reactions to drugs andor their solvents agents which PR histamine by nonspecific mechanisms are not uninteresting for the clinical situation | MEDAL |
the effect of the pph group in the antitumor activity of some new organometallic rutheniumii complexes has been investigated several complexes of the type ruiiclpphlign ruiicllign where lignpyridine derivate and ruiiclpph have been synthesized and characterized a noticeable increment of the antitumor activity and cytotoxicity of the complexes due to the presence of pph moiety has also been demonstrated affording ic values of μm in hl RT cell lines atomic force microscopy circular dichroism and PACE experiments have proved that these complexes can bind dna resulting in a distortion of both AA and tertiary structures ethidium bromide displacement fluorescence spectroscopy studies and viscosity measurements support that the presence of pph group induces intercalation interactions with dna indeed crystallographic analysis suggest that intramolecular ÏÏ interactions could be involved in the intercalation within dna base pairs furthermore high performance liquid chromatography mass spectrometry hplcms studies have confirmed a strong interaction between ruthenium complexes and proteins ubiquitin and potato carboxypeptidase inhibitor pci including slower kinetics due to the presence of pph moiety which could have an important role in detoxification mechanism and others finally ion mobility mass spectrometry imms experiments have proved that there is no significant NC in the gas phase structural conformation of the proteins owing to their bonding to ruthenium complexes | MEDAL |
the purpose of this T0 was to evaluate the variation in plasma adrenocorticotropic hormone acth concentration and dexamethasone suppression test dst results with season age and sex in healthy pony mares n and pony stallions n living under semiferal conditions and horse mares n living at pasture plasma acth concentrations were measured in september and in january may and september dsts were performed in january and september plasma acth concentrations in september and september were similar and were significantly greater than in january and may p plasma acth concentration was within the reference range for of subjects in january for of subjects in may for of subjects in september and for of subjects in september dst results were within the reference range in all subjects in january and were within the reference range for of subjects in september PCC at the end of the dst was significantly greater in september than in january p age was positively correlated with plasma acth and plasma cortisol concentration at the beginning and end of the dst within the same season plasma acth concentration in pony mares pony stallions and horse mares was not significantly different p seasonal changes in plasma acth concentration and dst results should be considered when interpreting endocrine test results | MEDAL |
the effects of dynamic exercise jumping and static overload synergist tenotomy on rat SOL were investigated during a growing phase in juvenile animals the relative proportions of slow twitch type fast twitch type a and intermediate type c fibers were determined histochemically the control animals as part developmental process lost type a fibers apparently by conversion to type SF via the type c form synergist tenotomy enhanced the conversion of type a fibers to type c SF as these muscles displayed fewer type a fibers than control animals at the same age in contrast the dynamic exercise delayed the loss of type a fibers from the soleus when the two exercises were combined the effects on fiber type proportions cancelled each other this group also had a C1 number of type c fibers this present study indicates that the activity imposed on a muscle influences the maturational development of the constituent fiber types | MEDAL |
TR of escherichia coli k is a dimeric protein monomer size amino acids that acquires high affinity for certain operator targets in dsDNA upon interaction with ltryptophan high titer AS directed against e coli trp repressor protein elicited in rabbits was monospecific toward native or denatured trp repressor using an enzymelinked immunosorbent CA to measure antigenantibody reaction we found that the binding of ltryptophan to trp repressor was associated with a marked decrease in antibody reactivity that presumably accompanied a conformational NC in this protein to a state with strong affinity for trp operatorbearing dna we analyzed the DP of cleavage of TR by chymotrypsin and trypsin and the effect of ltryptophan on such hydrolytic cleavages chymotrypsin cleaved trp repressor mainly between residues and in the presence of ltryptophan this cleavage was slowed the firstorder rate constants for chymotryptic digestion of TR were x and x min in the absence and presence of ltryptophan respectively tryptic digestion was more complex initial cleavage of trp repressor occurred with approximately equal facility between residues or subsequent tryptic hydrolyses led eventually to a major core fragment containing the first amino acids of trp repressor plus four other fragments from the carboxylterminal half of the protein in the presence of ltryptophan cleavage by trypsin between residues and was retarded even when a previous hydrolytic event elsewhere in the protein had occurred tryptophan had essentially no effect on the tryptic hydrolysis of peptide bond but accelerated cleavage at peptide bond the firstorder rate constants for the first tryptic cleavage of trp receptor were x and x min in the absence and presence of ligand respectively our results are compatible with a structural model wherein certain amino acid side chains and peptide bonds of trp repressor specifically those of residues lie on or near the surface of the protein this region of trp repressor has been predicted to contain the operator recognition site the susceptibility to proteolytic attack of at least four peptide bonds in this area changes when the protein interacts with ltryptophan | MEDAL |
the relationship between ET sevoflurane concentration bispectral index bis and the eeg bispectrum in children appears to be age dependent the aim of this study was to quantify the bis values at mac minimum alveolar concentration for desflurane and HAL and explore the relationship with age for these anaesthetic agents in children | MEDAL |
after birth a decrease in hemoglobin concentration occurs while high metabolic demands are imposed on the infant by the extrauterine environment using the resting lamb as a model we studied the mechanisms that are called into play during this period to maintain oxygen delivery measurements were made of oxygen consumption arterial and mixed venous blood oxygen contents cardiac SO hemoglobin concentration percent fetal hemoglobin diphosphoglycerate and hemoglobin P50 during the first two postnatal months there was a rapid decrease in hemoglobin concentration after birth and concomitant decrease in hemoglobin oxygen affinity changes similar to those described in humans cardiac output and oxygen consumption were both very high immediately T3 birth and declined in parallel so that arteriovenous oxygen content difference was constant thus at rest cardiac SO varies as a result of the changing need for oxygen this relationship is independent of hemoglobin concentration or P50 within the normal range if however oxygen demands were increased oxygen delivery might be compromised by a limited ability to increase oxygen SE during the immediate newborn period or when hemoglobin concentration is lowest | MEDAL |
complement factor a is a potent Th1 mediator that contributes to the pathogenesis of numerous inflammatory diseases proteinbased ca inhibitors have proven to be clinically valuable aptamers which are oligonucleic acid chains or polypeptides can bind to target molecules and hence have the potential to be used for detection and blockade of targets here we describe the discovery that the ssDNA aptamer s can bind specifically to swine ca which can then be quickly selected for with capillary electrophoresis for highthroughput sequencing aptamer s bound specifically to swine ca with a KB of μm as measured by SPR spr moreover aptamer s inhibited cainduced CTX of neutrophils in vitro our study suggests that the s aptamer has great potential to be a key structure in the OD of ERP therapeutic agents against inflammatory diseases | MEDAL |
the accessory ascending C2 i.a. murakami et al which arises from the subclavian artery and ascends between the scalenus anterior and medius muscles was studied in japanese AD cadavers sides with special attention being given to its origin CSD and relationship to other arteries at the C2 or scalenus region in sides the accessory ascending cervical artery was found to originate from the subclavian artery behind the scalenus anterior muscle and to branch out to the scalenus anterior and medius muscles as well as those entering the th and th intervertebral foramens along the th and th C2 SN this i.a. arose independently in sides the accessory ascending cervical artery issued off or formed a common trunk with the transverse cervical artery andor costocervical trunk in sides in cases lacking the accessory ascending C2 i.a. it was usually compensated for by the costocervial trunk andor transverse C2 artery sides common trunk formation with the vertebral internal thoracic or suprascapular arteries was not observed the authors suggest that the accessory ascending C2 i.a. the transverse cervical artery and the costocervical trunk should be grouped into one arterial system a system that may be a remnant of the precostal longitudinal anastomoses of intersegmental arteries of the dorsal aorta behind the scalenus anterior muscle | MEDAL |
polyglandular autoimmune syndrome pga and eosinophilic granulomatosis with polyangiitis egpa do not seem to represent a coincidental association | MEDAL |
recently we investigated the influence of CP and emerging antibiotics on the proteome of Bs including in our studies actinonin a potent novel inhibitor of peptide deformylase the protein synthesis pattern under actinonin treatment changed so dramatically that a direct comparison to the control DP was impossible dual channel imaging revealed that actinonin treatment caused the majority of newly synthesised proteins to accumulate in spots different from the ones usually observed indicating a more acidic isoelectric point two strategies were used to investigate the nature of the charge shift in the first place protein patterns of a conditional PDF mutant under nonrepressing and repressing conditions were compared secondly several protein pairs excised from 2DE d gels of the peptide deformylase mutant exponentially growing untreated wildtype and the actinonin treated wildtype were investigated with matrixassisted laser desorptionionization and ESI esi TOF mass spectrometry tof ms for the existence of nterminal formylation under nonrepressing conditions the mutant protein pattern resembled that of the wildtype the loss of peptide deformylase activity under repressing conditions led to the same pi shift observed for actinonin treatment in the wildtype quadrupole tofms on protein pairs proved that the remaining NT formyl residue was indeed responsible for the charge shift eight of these protein pairs were also present on d gels of exponentially growing b subtilis where the more acidic still formylated protein species represented the smaller parts | MEDAL |
fluorescence recovery after photobleaching was used to characterize the diffusion of fluorescently labeled phospholipids at the oilwater interface for oil viscosities that varied over four orders of magnitude measurements were performed over a range of surface concentrations corresponding to molecular Az of amolecule as expected the interfacial diffusion coefficient increased with molecular area saturating at an area of approximately amolecule at molecular areas below approximately amolecule macroscopic domains of a condensed monolayer phase were observed the diffusion of these domains was characterized by direct tracking and trajectory analysis for oils with viscosity cp the DWI coefficients of both individual probe molecules and condensed domains were consistent with a mechanism where the objects moved within the interface experiencing drag from the adjacent bulk phases because this drag was dominated by the oil viscosity the DWI coefficients decreased proportionally to the inverse of the oil viscosity however for oils with higher viscosity the diffusion coefficient of individual probe molecules decreased much more slowly these observations suggested that two diffusive mechanisms are involved one where surfactant molecules move within the interface and one that is analogous to the activated hopping processes that occur at the solidliquid interface this latter mode becomes significant only for very viscous oil phases | MEDAL |
avian cholera outbreaks have been identified in indonesia in recent years despite vaccination programs outbreaks continue to occur to date there has been a lack of information on the characteristics of pasteurella multocida isolates involved in these outbreaks hence the objective of this T0 was to characterize indonesian p multocida isolates in poultry during field outbreaks were reported in indonesia nine isolates of p multocida were recovered from these field outbreaks the isolates were compared with four vaccine strains that were used in indonesia and designated pmv pmv pmv and pmv the isolates were characterized by biotype capsular type somatic serotype restriction endonuclease analysis plasmid presence and antimicrobial susceptibility patterns of the nine indonesian isolates three were of capsular type a a a and a one isolate was of type b and one isolate was of capsular type f for three isolates the capsular serogroup could not be identified plasmids the size of kbp were present in three of the field isolates and two of the vaccine strains one plasmid less than kbp was isolated from the vaccine strain pmv eight distinct dna profiles were obtained from digestion with the restriction endonuclease ecori and seven distinct dna profiles were obtained from digestion with the restriction endonuclease hindiii all of the isolates were resistant to lincomycin and sulfadiazine and were susceptible to ampicillin and trimethoprim of the nine isolates seven were susceptible to doxycycline and gentamicin and six were susceptible to ENR | MEDAL |
the purpose of this study was to determine the effects of ST exercise cessation on lipid and lipoprotein profile and insulin sensitivity in highly trained runners n mean age years and SP athletes n mean age years following days of exercise cessation running time to exhaustion and maximal oxygen uptake decreased by and p in the runners while in the SP athletes one repetition maximum squat and bench press did not NC p no changes occurred in BC data from a h oral glucose tolerance test revealed an impairment of the glycemic state in all athletes p in contrast exercise cessation did not significantly p alter plasma C2 of cholesterol triglycerides and low density ldl and HD lipoprotein hdl no changes were observed in hdl hdlb and hdl subfractions ldl diameter and qualitative ldl DP p these data thus suggest that despite a decrease in insulin sensitivity shortterm exercise cessation independent of exercise mode was insufficient to alter plasma lipid and lipoprotein profiles in welltrained athletes | MEDAL |
we report a case of fatal pulmonary embolism pe developing T3 tympanoplasty a yearold woman underwent type iii tympanoplasty for a ME cholesteatoma under GA anesthesia operating time was hours minutes and anesthesia lasted hours T2 the next morning hours T2 after returning to the recovery room the patient lost consciousness while getting out of bed although consciousness recovered transiently she went into shock with cardiopulmonary arrest heart beat was regained after resuscitation with artificial respiration and cardiac massage but her blood pressure was unstable echocardiography revealed right ventricular overload and pulmonary hypertension because pe was suspected TT was conducted to stabilize hemodynamics enhanced computed tomography ct of the chest showed BL pulmonary thromboembolism the patient died of hypoxic HIVE days after pe onset we have seen cases of pe at our hospital in the last months five patients developed pe after surgery with a postoperative occurrence rate of and of them died enhanced ct in of cases before or just after the start of therapy for pe was useful in establishing the diagnosis although pe is rare in the field of otolaryngology and head and neck surgery it may develop rapidly after any type of surgery resulting in a fatal outcome it is thus important to establish diagnosis early and prevent such serious complications | MEDAL |
artificial SF alec made from dppc and upg ww has been given at birth to babies under weeks of gestation in three trials the first trial enrolled resuscitated babies treated with mg alec powder and controls the mortality fell from to p in the second trial babies were randomized to receive ml of mg alec suspension into the pharynx at birth and up to more ET doses in h controls received ml of saline there were alectreated babies and controls the mortality due to complications of respiratory distress syndrome rds fell from to p less than periventricular haemorrhage was reduced from to p less than the third trial involved babies randomized in centres alec treatment reduced the neonatal mortality from to p less than intracerebral haemorrhages fell from to p and severe rds by p less than SRA showed a significant reduction in hours receiving ventilation and greater than oxygen during the first days | MEDAL |
there is growing interest in quantitative analysis of in vivo genetic toxicity doseresponse data and use of pointofdeparture pod metrics such as the benchmark dose bmd for human HRA hhra currently multiple transgenic rodent tgr CA variants employing different rodent strains and reporter transgenes are used for the assessment of chemicallyinduced genotoxic effects in vivo however regulatory issues arise when different pod values eg lower bmd confidence intervals or bmdls are obtained for the same compound across different tgr CA variants this T0 therefore employed the bmd RPA to examine the ability of different tgr SCV to yield comparable genotoxic potency estimates review of over doseresponse datasets identified suitablymatched doseresponse data for three compounds ethyl methanesulfonate or ems nethylnnitrosourea or enu and dimethylnitrosamine or dmn across four commonlyused mu tgr variants mutaâ¢mouse lacz mutaâ¢mouse cii gpt delta and bigblue® laci DR analyses provided no conclusive evidence that tgr variant choice significantly influences the derived genotoxic potency estimate this conclusion was reliant upon taking into account the importance of comparing bmd confidence intervals as opposed to directly comparing pod values eg comparing bmdls comparisons with earlier works suggested that with respect to potency determination tissue choice is potentially more important than choice of tgr CA variant scoring multiple tissues selected on the basis of supporting toxicokinetic information is therefore recommended finally we used typical withingroup variances to estimate preliminary endpointspecific benchmark response bmr values across several tgr variantstissues we discuss why such values are required for routine use of genetic toxicity pods for hhra environ mol mutagen © her majesty the queen in RA of canada environmental and molecular mutagenesis published by wiley periodicals inc | MEDAL |
we used mri to examine the hips of asymptomatic patients at to months Tx covered by highdose corticosteroids five hips in three patients showed changes which indicate avascular necrosis although radiographs ct scans and isotope scans were normal these patients had repeat mri scans T3 another two years and three years one patient with bilateral mri changes developed symptoms and abnormal radiographs and ct and isotope scans in one hip nine months T3 the abnormal mri intraosseous pressure was found to be raised in both hips and core biopsies revealed necrotic bone on both sides the other three hips have remained asymptomatic with unchanged mri appearances three years after the initial mri it seems that idiopathic avascular necrosis does not always progress to bone collapse in the medium term | MEDAL |
this study examines the utility of the williams manual test wmt in predicting those older patients who will eventually require formal services one hundred seventeen older subjects underwent comprehensive geriatric assessment that included administration of the wmt and questionnaires assessing current service use T0 participants returned at and months for repeat measurement of study variables the wmt was found to be a significant predictor of additional service use at months chi p less than the test had high specificity but relatively low sensitivity the lack of sensitivity in this study group was likely due to high C2 of AS among caregivers whose relative was otherwise not predicted to need services when the wmt was repeated T3 and months the results were highly reproducible the wmt appears to be a useful predictor of increasing formal service use however in certain T0 groups its predictive ability may be affected by high C2 of caregiver stress | MEDAL |
we describe a new technique for arthroscopically assisted posterior cruciate ligament reconstruction we use the transfix arthrex naples fl pin to provir signalregulated kinase erk and akt kinase both of which protect against oxidant injury that reduced erk and akt MICs contribute to lower survival of aged cells was supported by additional findings first pharmacologic inhibition of erk and akt activation in young cells markedly increased their sensitivity to ho second caloric restriction which increases rodent life span and delays the ON of many agerelated declines in physiologic CF prevented loss in erk and akt activation by ho and enhanced survival of old hepatocytes to levels similar to those of young cells strategies aimed at boosting these host responses to acute oxidant injury could have significant antiaging benefits | MEDAL |
inflammation has been postulated to contribute to restenosis after balloon angioplasty RT necrosis factor TNF is a pleiotropic Th1 cytokine involved in many features of inflammation we examined the tissue expression DP of TNF and the inflammatory response to arterial injury and the effects of a goat antirabbittnfalpha polyclonal antibody on tissue tnfalpha expression inflammation and restenosis in a rb atherosclerotic MM at different time points following air dessication and subsequent balloon injury fresh rb FA artery tissues were homogenized and analyzed for tnfalpha levels by quantitative tnfalpha bioassay rabbits were treated with a goat antirabbittnfalpha polyclonal antibody SS and tissue tnfalpha neutralization macrophage infiltration as an indicator of inflammation and neointimal areas were determined balloon angioplasty increased tissue tnfalpha expression fold over baseline and this increase persisted over days T3 arterial injury SS antitnfalpha antibody C2 were sufficient to neutralize tissue TNF activity by macrophage infiltrati its possible consequence upon the PR of glucagon and other noninsulinic hormones by nonb islet cells do not appear sufficient to account for the greater enhancing action of cytochalasin b as distinct from cytochalasin d upon glucosestimulated insulin SO likewise the latter difference does not appear attributable to a greater efficiency of cytochalasin b as compared to cytochalasin d upon the mechanical events involved in nutrientstimulated exocytosis of insulin LG hence the present findings suggest a sofarunidentified interference of cytochalasin b with the bcell glucosesensing device | MEDAL |
arthropodborne flaviviruses are important human pathogens that cause a diverse range of clinical conditions including severe hemorrhagic syndromes neurological complications and congenital malformations consequently there is an urgent need to develop safe and effective vaccines a process requiring better understanding of the immunological mechanisms involved during infection decades of research suggest a paradoxical role of the immune response against flaviviruses although the immune response is crucial for the control clearance and prevention of infection poor clinical outcomes are commonly associated with virusspecific immunity and immunopathogenesis this relationship is further complicated by the high homology among viruses and the implication of CR immune responses in protection and pathogenesis this review examines the dual role of the adaptive immune response against flaviviruses particularly emphasizing the most recent findings regarding crossreactive t cell and antibody responses and the effects that these concepts have on vaccinedevelopment endeavors | MEDAL |
to determine whether danish patients want the opportunity to receive treatment with acupuncture during hospitalization we conducted a questionnaire survey among patients in the recovery room of the patients answered the questionnaire were interested in receiving treatment with acupuncture for nausea and pain out of these were interested in receiving treatment with AP before ordinary medical therapy and were interested in receiving acupuncture as a supplement to conventional therapy throughout their admission at the hospital | MEDAL |
familial hypertrophic cardiomyopathy fhc is a leading cause of sudden cardiac death among young athletes but the PET effects of the myofilament mutations during fhcassociated ischemia and acidosis due in part to increased extravascular compressive forces and microvascular dysfunction are not well characterized we tested the hypothesis that the fhclinked TM tm mutation tmeg alters the contractile response to acidosis via increased myofilament ca sensitivity intact papillary muscles from transgenic tg mice expressing tmeg and exposed to acidic conditions ph exhibited a significantly smaller decrease in normalized isometric tension compared to nontransgenic ntg S9 times to peak tension and to of Pt relaxation in tg PTC muscles were significantly prolonged IN single ventricular tg myocytes demonstrated significantly less inhibition of unloaded shortening during moderate acidosis ph than ntg myocytes the peak ca transients were not different for tg or ntg at any ph tested the TC of relengthening was slower in tg myocytes but not the rate of ca decline tg detergentextracted fibers demonstrated increased ca sensitivity of force and maximal tension compared to ntg at both normal and acidic ph ph tm phosphorylation was not different between tg and ntg muscles at either ph our data indicate that acidic ph diminished DF in hearts of tg mice less than in ntg due to their inherently increased myofilament ca sensitivity thus potentially contributing to altered SE demands and increased propensity for contractile dysfunction | MEDAL |
in term and PT neonates massive glu release can lead to excitotoxic whitematter and cortical lesions because of its high permeability toward calcium the nmethyldaspartic acid nmda receptor is thought to play an important role in excitotoxic lesions and nmda antagonists therefore hold promise for neuroprotection we found that in neonatal mouse SC a given nmda concentration exerted either excitotoxic or Bcl-2 effects depending on the cortical layers in SL vi nmda led to excitotoxicity sustained calcium mobilization and necrosis of gadgfp neurons in the immature layers iiiv nmda decreased apoptosis and induced transient calcium mobilization the nmda antagonist mk acted as a potent caspase activat has the potential to address longerterm needs of participants | MEDAL |
we have shown previously that a chimeric fusion protein composed of human interleukin il and pseudomonas exotoxin pe termed il cytotoxin ilpe is specifically cytotoxic to various CA cell lines and primary cell cultures derived from a variety of solid cancers in addition we have shown that il mutant ilek in which glu e residue at position of il molecule was substituted by a lysine k is a powerful antagonist of il and binds to il receptor with a higher affinity compared with wildtype il in this T0 we have generated an il cytotoxin ilekpe in which il antagonist is fused to pe to determine whether this molecule has improved cytotoxicity to RT cells compared with wild type wtilpe highly purified ilekpe was tested in various tumor cell lines including seven glioblastoma multiforme cell lines to compare its IB to the cells in vitro cytotoxicity in vivo antitumor activity and safety in mouse MM with wtilpe ilekpe bound to umg and ilralpha chaintransfected RT cell lines with to times higher affinity compared with wtilpe however ilekpe did not show higher cytotoxicity compared with wtilpe in glioblastoma multiforme or any other cell lines tested the antitumor activity of ilekpe when po intraperitoneally to nude mice bearing u tumors was also similar to wtilpe some improvement in antitumor activity was observed when lower doses of ilekpe were injected intratumorally in subcutaneous PT these results indicate that in general ilekpe mediates similar cytotoxicity and antitumor activity to wtilpe despite its improved IB affinity to IL-2R | MEDAL |
the human visual system appears to be capable of temporally integrating information in a video sequence in such a way that the perceived spatial resolution of a CS appears much higher than the spatial resolution of an individual frame while the mechanisms in the human visual system that do this are unknown the effect is not too surprising given that temporally adjacent frames in a video sequence contain slightly different but unique information this paper addresses the use of both the spatial and temporal information present in a short image CS to create a single highresolution video frame a novel observation model based on motion compensated subsampling is proposed for a video CS since the reconstruction problem is illposed bayesian restoration with a discontinuitypreserving prior image model is used to extract a highresolution video still given a short lowresolution CS estimates computed from a lowresolution image sequence containing a subpixel camera pan show dramatic visual and quantitative improvements over bilinear cubic bspline and bayesian single frame interpolations visual and quantitative improvements are also shown for an image sequence containing objects moving with independent trajectories finally the video frame extraction algorithm is used for the motioncompensated scan conversion of interlaced video data with a visual comparison to the resolution enhancement obtained from progressively scanned frames | MEDAL |
we propose to study the scattering properties of dense distributions of spherical scatterers by resorting to an iterative solution of the foldytwersky equation for the propagation of the coherent field as a result of the first step of the iterative procedure the host medium is substituted by an effective medium of complex refractive index to account for the multiplescattering processes that occur among the particles although we truncate the abovementioned iterative procedure to the second step the results of our calculations are in excellent agreement with previous experimental results of zaccanti et al measurement of optical properties of highdensity media to be published in applied optics for the scattering coefficient of intralipid solutions up to a volume density of and show a limited disagreement at a volume density of | MEDAL |
cells involved in immune responses inflammation and development are sensitive to interleukin il in recent years many studies have addressed the effects of il on carbohydrate metabolism in vivo studies in rats and mice have shown that il induces hypoglycemia with or without changes in serum insulin levels according to the species the genesis of this effect has been investigated by studying CNS system CL hepatocyte and IP tissue responses to il in vitro betaislet cells incubated briefly with il produced insulin incubation for longer periods of time or with high levels of il induced cytotoxic effects for which suggested mechanisms include production of FRs alterations in mitochondrial CF increased production of phosphoinositide second messenger accumulation of prostaglandins alterations in ion fluxes or changes in gene transcription studies of links between il and diabetes mellitus have focused on animal models of genetic diabetes including the non obese diabetic nod mouse and the biobreeding bb rat although currently available data suggest that il modulates the incidence of diabetes mellitus they are not adequate to determine whether or not il has a potential for preventing development of the disease | MEDAL |
the equilibrium eq score commonly used in computerized dynamic posturography is normalized between and with falls assigned a score of the resulting mixed discretecontinuous CSD limits certain statistical analyses and treats all trials with falls equally we propose a simple modification of the formula in which peaktopeak sway data from trials with falls is scaled according the percent of the trial completed to derive a continuous equilibrium ceq score the ceq scores for trials without falls remain unchanged from the original methodology the ceq factors in the time before a fall and results in a continuous variable retaining the CE tendencies of the original eq CSD a random set of sensory organization test trials were pooled that included falls a comparison of the original and ceq distributions and their rank ordering demonstrated that trials with falls continue to constitute the lower range of scores with the ceq methodology the area under the receiver operating characteristic curve demonstrates that the ceq retained nearperfect discrimination between trials with and without falls we conclude that the ceq score provides the ability to discriminate between ballistic falls from falls that occur later in the trial this RPA of incorporating time and sway magnitude can be easily D2 to enhance other balance tests that include fall data or incomplete trials | MEDAL |
thoracic electrical bioimpedance is a noninvasive continuous method of obtaining cardiac output that requires no operator skill however the most recent TEB technology has not been validated in pregnancy we therefore compared two methods of measuring cardiac SO in pregnancy thoracic electrical bioimpedance and TD we studied patients who required PAC for peripartum management and measured cardiac SO simultaneously by thoracic electrical bioimpedance and thermodilution among eight of nine patients there was agreement within between the two methods bivariate LR with these nine cases showed excellent correlation r p less than with a slope of which indicated a onetoone relationship between TEB and TD the remaining two cases were removed from analysis because of septic shock in one case which invalidates TEB and TR in another case which invalidates thermodilution these data support that TEB measurement of cardiac output may be valid in most peripartum patients | MEDAL |
one hundred and five patients with RA arthritis treated with a variety of antirheumatic drugs excepting GCs were stratified according to the degree of functional impairment functional classes i to iv and duration of the disease years years and greater than years the variables investigated were distal forearm bone mineral content bmc PSA markers of bone formation SS ALP phosphatase and SS bone Gla containing protein bgp and biochemical markers of bone resorption fasting urinary calcium and fasting urinary hydroxyproline significant relationships were found between bmc and PET impairment and duration of the disease indices of bone formation and bone resorption rose with increasing functional impairment particularly those of bone resorption it is concluded that disability induces osteopenia in rheumatoid ICA by increasing the bone turnover with a more marked increased in resorption than in the formation processes the effect of the disease duration is merely that of adding more years of PET impairment | MEDAL |
two male travelers with histories of gout and hazardous alcohol consumption presented with a triad of severe culturepositive disseminated gonococcal infection crystalpositive polyarticular gout and GC soft tissue collections following unprotected sexual contact in the philippines both men initially attributed symptoms to gout since their usual joints were affected but clinical deterioration occurred with selfadministration of antiinflammatory agents CT the clinical courses were severe and protracted requiring aggressive management of infection with prolonged intravenous antimicrobials and repeated surgery and prolonged antiinflammatory agents for gout joint symptom ON in each case occurred within a week of sexual SE in conjunction with hazardous alcohol ingestion we speculate that acute dissemination of infection to previously damaged joints triggered polyarticular gout with progressive infection exacerbated by unopposed antiinflammatory agents and delayed antibiotics disseminated GC infection can occur with polyarticular gout and delays in recognition and treatment including while traveling can lead to severe disease from both | MEDAL |
advances in FL protein OD over the past years have led to finetuning of the aequorea victoria jellyfish color palette in the emission color range from blue to yellow while a significant amount of progress has been achieved with reef coral species in the generation of monomeric FPs emitting in the orange to farred spectral regions it is not inconceivable that nearinfrared fluorescent proteins loom on the horizon expansion of the fluorescent protein family to include optical highlighters and fret biosensors further arms this ubiquitous class of fluorophores with biological probes capable of photoactivation photoconversion and PCD of molecular interactions beyond the resolution limits of OM the success of these endeavors certainly suggests that almost any biological parameter can be investigated using the AGA fluorescent proteinbased application | MEDAL |
a method was developed for the CA of the cflectin factor in amniotic fluid using samples from fetuses identified by other means as having or not having cystic fibrosis cf the method involves protein removal from the amniotic fluid by filtration through centricon amicon filters admixture of filtrate with normal SS as a source of igm and incubation in dextransulfate with mouse red blood cells to detect TPS hemagglutination blind samples submitted from four different laboratories were used to evaluate the ability of the assay to differentiate cf fetuses from fetuses without cf storage of samples from one laboratory had been quite prolonged and was associated with poor correlations freezing and thawing of samples were shown to deactivate the cflectin factor in three of the four studies cf lectin was detected in of pregnancies identified as cf at birth prenatally through microvillar enzyme CA or through dna analysis of terminated pregnancies eightythree percent of samples identified as normal were confirmed as such by a lack of cflectin activity these data suggest that cf fetuses produce and are exposed to the cflectin factor at least as early as the second trimester of gestation | MEDAL |
PTX a new nonclassical antifolate was evaluated in a multiinstitutional phase i trial in children the starting dose was mgmday administered po every h for consecutive days with courses repeated every days dose reduction initially to mgmday and subsequently to mgmday was required because dose limiting myelosuppression and mucositis were encountered at the and mgmday DLs nondose limiting toxicities included transient elevations in CL CF tests mild nausea and skin rashes the maximum tolerated dose was mgmday for days pharmacokinetic monitoring was performed at Css during the first course for the and mgmday dose CG the mean se peak PC were and microm respectively and occurred at a median of h following the po dose the mean area under the plasma concentrationtime curves were and micromh respectively absolute bioavailability in two patients who were also monitored following a single iv dose of and mgmday of piritrexim was and respectively dose limiting toxicities were observed in of patients with h trough PTX concentrations greater than microm whereas only of patients with trough concentrations less than microm experienced dose limiting toxicities a limited pharmacokinetic sampling strategy that allowed the area under the plasma concentrationtime curve to be accurately predicted from the and h plasma drug concentration was developed the recommended dose for future phase ii trials is mgmday po po every h for consecutive days pharmacokinetic monitoring at and h postdose may be useful for estimating bioavailability and for predicting which patients are at greatest risk for developing toxicity | MEDAL |
several human rhabdomyosarcoma cell lines cultured primary RT explants and biopsies of tumor and normal skeletal muscle tissue expressed a kilobase transcript that hybridized to the mouse muscle determination gene myod this transcript was found in RT cell lines and primary explants that developed multinucleated myotubes but was absent in wilms tumors or cell lines and primary explants that developed multinucleated myotubes but was absent in wilms tumors or cell lines derived from other mesenchymal RT cell types expression of the human homolog of myod therefore can define a tumor as a rhabdomyosarcoma transfection of the mouse myod gene into the human rhabdomyosarcoma cell L1 rd increased the ability of the tumor cells to differentiate into multinucleated myotubes and enhanced S1 heavychain mRNA but did not decrease tumorigenicity in nude mice | MEDAL |
an automated method for detecting and counting eye movements using the electrooculogram eog wave form during rapideyemovement rem sleep is presented the method is formulated as a sequential decision process with decisions based on slope and amplitude threshold criteria signal processing techniques such as digital filtering and smoothing are used to improve the effectiveness of the method validation is done by using the method on eog data from three infants and comparing the automated count of eye movements with the visual counts of human observers the rho coefficient between the automated and visual count is greater than the firstorder regression coefficient close to and the zeroorder beta close to we believe that this method will be useful in differentiating between the substates of rem sleep in studies of CR physiology | MEDAL |
the presence of tumor thrombus in the right atrium is frequently the result of direct intraluminal extension of infradiaphragmatic malignancy into the inferior vena cava ivc or supradiaphragmatic carcinoma into the superior VC svc RA atrial tumor thrombus with extension into both svc and ivc has not been reported in the literature we present a patient who presented with PS of RA atrial and svc one imaging revealed presence of a thrombus in the RA atrium extending to the svc and ivc with the additional findings of a left adrenal mass and multiple CL lesions the histopathological examination of the RA atrial mass revealed metastatic adenocarcinoma cells the patient was given a presumptive diagnosis of metastatic adenocarcinoma most likely adrenal in origin with multiple hepatic lesions suspicious for metastasis the clinical outcome of the patient was not favorable the patient succumbed before the adrenal mass could be confirmed to be the primary RT this case highlights that in patients manifesting with extensive cavoatrial thrombus as the existence of primary carcinoma should be considered especially in the adrenal SC or in the lung | MEDAL |
chromosomal translocation occurs in some CA cells resulting in the expression of aberrant oncogenic F0 proteins that include bcrabl in chronic myelogenous leukemia cml inhibitors of abl IMT kinase such as imatinib and dasatinib exhibit remarkable therapeutic effects although emergence of drug resistance hampers the therapy during LT treatment an alternative approach to treat cml is to downregulate expression of the bcrabl protein recently we have devised a protein knockdown system by hybrid molecules named specific and nongenetic inhibitor of apoptosis protein iapdependent protein erasers sniper this system is designed to induce iapmediated ubiquitylation and proteasomal degradation of target proteins in this review we describe the development of sniper against bcrabl and discuss the features and prospect for treatment of cml | MEDAL |
early diagnosis and treatment of sepsis by appropriate antibiotics is of utmost importance therefore we evaluated s rrna panbacterial polymerase chain reaction pcr for rapid diagnosis of sepsis in AD patients in intensive care units icus and compared it with an automated blood culture ml of ml blood collected was inoculated into bactec® aerobic bottle and the remaining ml was used for dna SE and pcr of episodes of suspected sepsis showed concordant results between automated culture and pcr six episodes were positive by pcr only panbacterial pcr reduces TAT with rapid differentiation between systemic inflammatory response syndrome and sepsis | MEDAL |
employing a structural equation model to evaluate a measurement scale can be challenging especially for a multidimensional scale that contains many items we describe two issues that can contribute to the poor fit of such models the statistical power associated with the test of a C1 measurement scale and the degree of rho between items and factors within the scale these issues are not well understood so our purpose is to explain them at an applied level clarify their practical implications for tests of measurement scales and other large structural equation models and discuss potential strategies for addressing them | MEDAL |
five tests of autonomic function were evaluated in a group of twentyfive diabetics the VR proved to be the most valuable single indicator of generalised AD whilst the blood pressure response to sustained handgrip the pupillary response to a dilute solution of methacholine and the fall in blood pressure on standing all proved to be less valuable as indices of AN either when considered individually or when taken together the CPT was shown to be of no value in the assessment of patients with autonomic neuropathy it is suggested that of those examined the valsalva procedure is the most valuable simple outpatient test for autonomic neuropathy | MEDAL |
previous cross sectional studies on potential neurotoxic effects of LT aluminium exposures by Al welders lack clear interpretable results for methodological reasons the present longitudinal T0 examined on the one hand the reliability and representativity of albiomonitoring as indicator of individual LT exposure and on the other hand the LT changes of neurobehavioural performance in al welders in relation to al exposure and neurobehavioural performance changes of a nonexposed control group | MEDAL |
interferonalphab ifnalphab is the only form of systemic adjuvant therapy for stage iii melanoma with documented survival benefit radiotherapy can also be utilized in the adjuvant setting in patients at high risk of nodal basin recurrence as ifnalphab is associated with substantial toxicity we sought to determine both the SVR and radiationrelated toxicities in patients treated with combined adjuvant ifnalphab and regional adjuvant radiotherapy delivered in the setting of a single institution eighteen consecutive patients who commenced adjuvant ifnalphab between november and august were analysed retrospectively for toxicities associated with the combination of ifnalphab and adjuvant radiotherapy gy in fractions to nodal basins delivered during the maintenance phase of ifnalphab therapy median dose during radiotherapy of mum three times per week seven out of patients who received concurrent radiotherapy and ifnalphab displayed grade skin reactions severe radiationinduced toxicity was seen in three further patients one who developed radiation pneumonitis one who developed severe OM and one who developed wound dehiscence that took months to resolve nonradiationrelated toxicity to ifnalphab therapy was typical for this dose and schedule we conclude that concurrent use of adjuvant radiotherapy and ifnalphab may enhance radiationinduced toxicity however overall we found concurrent radiation and ifnalphab could be safely delivered with AGA clinical monitoring | MEDAL |
the autobiographical implicit association test aiat is a variant of the implicit association test reportedly capable of detecting an individuals concealed autobiographical event with very high accuracy a previous attempt to utilize this measurement technique for the identification of cocaine users rendered an alarming rate of false positives in this T0 we aimed to explore the potential reasons behind the measurements inaccuracy | MEDAL |
we have previously shown that while ischemic preconditioning and CP afford similar protection against injury during ischemia and reperfusion this protection is not additive however it is not known whether the same applies when the delivery of cardioplegia is suboptimal such as may occur in the case of a CS to investigate the protective effect of cardioplegia v preconditioning when the delivery of cardioplegia is impaired isolated rat NF were subjected to min of GI followed by IR seven groups of NF g wet wt were studied n group group controls with unprotected ischemia no IG in which only ml of the st thomas cardioplegic solution was po min prior to ischemia group in which the volume of CPS was increased to ml group received ml of CP group received ml of CP group were subjected to ischemic preconditioning min ischemia min reperfusion min ischemia min reperfusion prior to ischemia and group in which ischemic preconditioning and cardioplegia ml were used in combination the mean postischemic recovery of left ventricular developed pressure lvdp expressed as a percentage of its preischemic value was in the control group this was significantly improved p in NF receiving ml of CP whereas volumes of or ml of cardioplegia afforded no significant protection recoveries of lvdp were and respectively preconditioning alone and the combination of preconditioning plus ml of CP afforded similar protection and p v controls at the end of reperfusion left ventricular EDV pressure lvedp was substantially increased in control NF mmhg it was decreased in the CG receiving ml of cardioplegic solution mmhg p v control group but not in the group receiving or ml of CPS and mmhg respectively again preconditioning alone or preconditioning plus ml of cardioplegia afforded similar good protection mmhg and mmhg respectively a similar DP was observed for the postischemic REC of CF under conditions where the delivery of cardioplegia is impaired mlg myocardium preconditioning CT or preconditioning in combination with CP is more protective that CP CT these results may be of clinical interest because most patients undergoing surgery for ischemic heart disease suffer from severe CAL that may prevent the delivery of sufficient cardioplegic solution to ensure maximum protection | MEDAL |
a versatile oral controlled release system for the simultaneous delivery of LD methyl ester and carbidopa consisting of a threelayer matrix tablet has been studied and developed each individual layer of the matrix exhibited a different release mechanism ie the first layer was swellable s the second one was erodible e and the third one was disintegrating d the three layers have been assembled in the monolithic matrix in different relative positions it was found that in the monolith the three layers could interact producing in vitro release profiles depending on their relative position the monoliths having the configurations dse and sde were administered to human volunteers in order to determine the plasma profiles the pharmacokinetic data showed a significant difference between the early time plasma curves the monolith dse having the fast release profile gave rise to a rapid appearance of a high levodopa plasma level whereas the slower releasing monolith sde produced a smoothed plasma concentration profile | MEDAL |
the purpose of this T0 was to analyse the effects on gastrooesophageal reflux gor of the different manipulations used during pulmonary PCS therapy nasal suctions oropharyngeal suctions acceleration of expiratory flow children less than year old were divided into two CG according to the results of oesophageal EPM with and without pathological gor nasal suctions did not significantly increase gor in children without pathological gor OP suctions increased gor significantly more than nasal suctions p the technique of acceleration of expiratory flow used in degrees verticalized position and SP increased the index of reflux and the time with oesophageal ph below in children with pathological gor however it did not increase reflux in normal children the index of induced reflux in the whole population was increased in the horizontal position refluxhour as compared with degrees verticalized refluxhour these oesophageal ph measurements confirm the clinical observations often made during PT vomiting regurgitations reflex cough this statistical study suggests that one should take into account the adverse factor of gor secondary to pulmonary PT it seems better to use nasal rather than oropharyngeal suctions and also to use the acceleration of expiratory flow technique in the degrees verticalized rather than in the SP even more so if a pathological reflux does exist | MEDAL |
interferongamma can facilitate the spinal nociceptive FR and may elicit neuropathic painrelated SMB in rats and mice immunoreactivity for the interferongamma receptor ifngamma r occurs in the superficial layers of the DH and the LSN in the rat and mouse spinal cord as well as in subsets of neurons in the dorsal root ganglia the aim of the present study was to examine the cellular localization and origin of the Th1 r in the spinal cord as viewed by confocal microscopy the immunopositivity for the Th1 r was colocalized with that of the presynaptic marker synaptophysin and with neuronal nitric oxide synthase in the LSN whereas only a minor overlap with these molecules was observed in laminae i and ii of the dorsal horn there was no colocalization of the Th1 r with markers for astrocytes and microglial cells ultrastructurally the Th1 r was found predominantly in axon terminals in the lateral spinal nucleus but at postsynaptic sites in dendrites in laminae i and ii the Th1 r expressed in SN in dorsal root ganglia was transported in axons both centrally and peripherally hemisection of the spinal cord caused no reduction in immunolabelling of the ifngamma r in the DH or the lateral spinal nucleus since rhizotomy does not affect the immunolabelling in the lateral spinal nucleus our observation indicates that the presynaptic receptors in this nucleus are derived from intrinsic neurons the localization of the ifngamma r in the spinal cord differed from that of the ampa glu receptor subunits and and the NK1 nk our results showing localization of Th1 r to pre and postsynaptic sites in the DH and LSN indicate that ifngamma can modulate nociception at the spinal cord level | MEDAL |
large scale tetragonal nalamoo nanocrystallines with spindle morphology have been prepared through a facile method by lowtemperature hydrothermal treatment of namoo lacl and edta in an ethanolaminewater mixed solution the products were characterized by xray powder diffraction fieldemission SEM microscopy highresolution transmission electron microscopy and PL spectroscopy results indicate that highquality and uniform nanospindles with a mean length of nm and a mean width of nm can be easily obtained furthermore edta and ea ethanolamine were found to have great influence on the formation of nalamoo spindlelike nanostructures the RT pl spectrum of the products shows a relatively strong emission peak at nm | MEDAL |
surgically assisted rapid maxillary expansion is performed to correct transverse deficiencies of the maxilla and it is indicated in TPS clinical situations the literature presents different opinions in several aspects mainly regarding the effect of disjunction of the pterygoid plates the aim of this T0 was to evaluate the DP of maxillary expansion obtained with two surgical techniques with and without disjunction of the pterygoid plates | MEDAL |
upon IB hormones or drugs many g proteincoupled receptors are internalized leading to receptor recycling receptor desensitization and downregulation much less understood is whether heterotrimeric g proteins also undergo agonistinduced endocytosis to investigate the intracellular trafficking of g alpha s we developed a PET g alpha sgreen fluorescent protein gfp fusion protein that can be visualized in living cells during signal transduction c and mcf cells expressing g alpha sgfp were treated with microm isoproterenol and trafficking was assessed with FM upon isoproterenol stimulation g alpha sgfp was removed from the BPM and internalized into LDV LDV containing g alpha sgfp did not colocalize with markers for early endosomes or late endosomeslysosomes revealing that g alpha s does not traffic through common endocytic pathways furthermore g alpha sgfp did not colocalize with internalized betaadrenergic receptors suggesting that g alpha s and receptors are removed from the plasma membrane by distinct endocytic pathways nonetheless activated g alpha sgfp did colocalize in vesicles labeled with fluorescent cholera toxin b a lipid raft marker agonist significantly increased g alpha s protein in triton x insoluble MF suggesting that g alpha s moves into lipid raftscaveolae after activation disruption of raftscaveolae by treatment with cyclodextrin prevented agonistinduced internalization of g alpha sgfp as did overexpression of a dominantnegative dynamin taken together these results suggest that receptoractivated g alpha s moves into lipid rafts and is internalized from these membrane microdomains it is suggested that agonistinduced internalization of g alpha s plays a TPS role in g proteincoupled receptormediated signaling and could enable g alpha s to traffic into the cellular interior to regulate effectors at multiple cellular sites | MEDAL |
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